What is autoinduction in protein expression?

Autoinduction is a simple approach for protein expression that needs little user intervention after inoculation of the culture (Studier, 2005).

How does autoinduction media work?

The principle of autoinduction media is based on carbon sources in the medium that are metabolized differentially to promote high density cell growth and automatically induce protein expression driven by lac promoters.

What is autoinduction media?

Auto Induction Media (AIM) have been formulated to grow IPTG-inducible expression strains, initially without induction, and then to induce production of target protein automatically, usually near saturation at high cell density.

What is Autoinduction of metabolism?

↵1 Autoinduction is defined as the ability of a drug to induce enzymes that enhance its own metabolism, resulting in dispositional tolerance.

What can BL21 chemically competent cells be used for?

BL21 competent cells are an all-purpose strain for high-level protein expression and easy induction. The basic BL21 strain does not contain the T7 RNA polymerase gene and can be used with non-T7 RNA polymerase protein expression systems. BL21 has the tightest control of protein expression for extremely toxic proteins. For Research Use Only.

Is the BL21 strain a T7 gene?

The basic BL21 strain does not contain the T7 RNA polymerase gene and can be used with non-T7 RNA polymerase protein expression systems. BL21 has the tightest control of protein expression for extremely toxic proteins. For Research Use Only. Not for use in diagnostic procedures.

What kind of RNA polymerase does BL21 contain?

The basic BL21 strain does not contain the T7 RNA polymerase gene and can be used with non-T7 RNA polymerase protein expression systems. BL21 has the tightest control of protein expression for extremely toxic proteins. For Research Use Only. Not for use in diagnostic procedures. Items were successfully added to your cart!