What are the reagents in the PCR beads?

PCR Beads have been optimized for PCR reactions and contain buffer, nucleotides and Taq DNA Polymerase. The only reagents that must be added to the reaction are template DNA and specific primers.

What is in a PCR Edvotek bead?

PCR EdvoBeads provide the reagents for PCR reactions in a convenient ambient-temperature-stable bead. PCR Beads have been optimized for PCR reactions and contain buffer, nucleotides and Taq DNA Polymerase. The only reagents that must be added to the reaction are template DNA and specific primers. 1.

What are the ingredients that go into the PCR tube?

The key ingredients of a PCR reaction are Taq polymerase, primers, template DNA, and nucleotides (DNA building blocks). The ingredients are assembled in a tube, along with cofactors needed by the enzyme, and are put through repeated cycles of heating and cooling that allow DNA to be synthesized.

What chemicals can be found in the PCR buffer?

The major components of PCR buffer include Tris-HCl, potassium chloride (KCl) and magnesium chloride (MgCl2). Tris-HCl and KCl are responsible for maintaining a stable pH during PCR. Magnesium ions act as cofactors for DNA polymerase so as to ensure proper DNA synthesis function of the polymerase during PCR.

What is the purpose of PCR beads?

These beads are preformulated to provide greater reproducibility between reactions, minimize pipetting steps, and reduce the potential for pipetting errors and contamination. The only additional reagents required are water, primers, and template DNA. The beads come predispensed into either 0.2-mL or 0.5-mL PCR tubes.

What is the purpose of PCR beads in the reaction?

These beads are preformulated to provide greater reproducibility between reactions, minimize pipetting steps, and reduce the potential for pipetting errors and contamination. The only additional reagents required are water, primers, and template DNA.

What must the PCR bead contain to perform a PCR experiment?

To make the PCR reaction more simple to prepare, we will be using Ready-to-Go PCR beads from GE Healthcare. These beads contain buffer salts, nucleotides and Taq polymerase in a lyophilized powder. All that must be added is water, primer and template to a total volume of 25 µl.

What are the four ingredients needed for PCR?

What 5 components are needed for PCR?

What components of PCR are included in the Ready to Go PCR?

The following components are included in this product: puReTaq Ready-To-Go PCR Beads: Room temperature-stable beads containing stabilizers, BSA, dATP, dCTP, dGTP, dTTP, ~2.5 units of puReTaq DNA polymerase and reaction buffer.

How are puretaq ready to go PCR beads made?

The PuReTaq Ready-To-Go PCR Beads are pre-formulated to ensure greater reproducibility between reactions. The only additional reagents required are water, primers, and template DNA. The beads are provided pre-dispensed into either 0.2 mL or 0.5 mL PCR tubes.

Why do we need RTG PCR bead solutions?

For a particular application, RTG PCR bead solutions that fit seamlessly into your workflows can be developed, often saving you time and money. The product can be provided that is ready to use with little or no need for further modification allowing you to focus on your research.

How many PCR beads are in a 0.2 ml tube?

The beads are provided pre-dispensed into either 0.2 mL or 0.5 mL PCR tubes. The 0.2 mL tubes are also supplied in a 96-well (8 × 12) plate format that allows individual strips of eight tubes to be easily removed. This flexibility allows use of either the entire 96-well plate, strips of eight, or individual 0.2 mL tubes.