What are Phasmid vectors?

A phagemid or phasmid is a DNA-based cloning vector, which has both bacteriophage and plasmid properties. These vectors carry, in addition to the origin of plasmid replication, an origin of replication derived from bacteriophage.

What is phage vector?

Phage vectors consist of an essentially complete phage genome, often M13 phage, into which is inserted DNA encoding the protein or peptide of interest (Figure 1). Typically, the remainder of the phage genome is left unchanged and provides the other gene products needed for the phage life cycle.

What is PAC vector?

PAC VECTOR OR PHAGE P1 VECTOR  Phage P1 is a temperate bacteriophage which has been extensively used for genetic analysis of Escherichia coli because it can mediate generalized transduction.  Sternberg and co-workers have developed a P1 vector system which has a capacity for DNA fragments as large as 85 – 100 kb. (

What is phagemid Slideshare?

Phagemids/Phasmids: plasmid/F1M13 hybrids A phagemid or phasmid is a plasmid that contains an f1 origin of replication from an f1 phage.  Phagemids contain an origin of replication (ori) for double stranded replication, as well as an f1 ori to enable single stranded replication and packaging into phage particles.

Which is the best description of a phagemid vector?

PHAGEMID VECTORS A phagemid or phasmid is a type of cloning vector developed as a hybrid of the filamentous phage M13 and plasmids to produce a vector that can grow as a plasmid, and also be packaged as single stranded DNA in viral particles. Phagemids contain an origin of replication (ori) for double stranded replication,…

Which is PowerPoint presentation uses Ti plasmid based vectors?

Lecture 3 Ti plasmid derived vector system Ti Plasmid Ti-plasmid based vectors Co-integrated vectors (hybrid ti-plasmids) Ti plasmid vector systems are often working – PowerPoint PPT presentation PowerShow.com is a leading presentation/slideshow sharing website.

How is a phagemid different from a plasmid?

PHAGEMID VECTORS A phagemid or phasmid is a type of cloning vector developed as a hybrid of the filamentous phage M13 and plasmids to produce a vector that can grow as a plasmid, and also be packaged as single stranded DNA in viral particles.

What can a pBluescript 2 phagemid be used for?

INTRODUCTION The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.