How do metalloproteinases work?

A member of a group of enzymes that can break down proteins, such as collagen, that are normally found in the spaces between cells in tissues (i.e., extracellular matrix proteins). Because these enzymes need zinc or calcium atoms to work properly, they are called metalloproteinases.

What is the function of matrix metalloproteinases?

Matrix metalloproteinases (MMPs), also called matrixins, function in the extracellular environment of cells and degrade both matrix and non-matrix proteins.

How are MMPs inactivated?

The MMPs are inhibited by specific endogenous tissue inhibitor of metalloproteinases (TIMPs), which comprise a family of four protease inhibitors: TIMP-1, TIMP-2, TIMP-3, and TIMP-4. Synthetic inhibitors generally contain a chelating group that binds the catalytic zinc atom at the MMP active site tightly.

How is base catalysis carried out in matrix metalloproteinase?

In the first mechanism, Browner M.F. and colleagues proposed the base-catalysis mechanism, carried out by the conserved glutamate residue and the Zn 2+ ion. In the second mechanism, the Matthews-mechanism, Kester and Matthews suggested an interaction between a water molecule and the Zn 2+ ion during the acid-base catalysis.

What kind of enzyme is a metalloproteinase?

Jump to navigation Jump to search. A metalloproteinase, or metalloprotease, is any protease enzyme whose catalytic mechanism involves a metal.

What is the role of metalloproteinase in tissue remodeling?

Matrix metalloproteinases combines with the metal binding protein, metallothionine; thus helping in metal binding mechanism. The MMPs play an important role in tissue remodeling associated with various physiological or pathological processes such as morphogenesis, angiogenesis, tissue repair, cirrhosis]

How does the pro-domain of a matrix metalloproteinase work?

Removal of the pro-domain enables access of a catalytic solvent molecule and substrate molecules to the active-site cleft, which harbors a hydrophobic S 1′ -pocket as main determinant of specificity. Together with the catalytic zinc ion, this pocket has been targeted since the onset of drug development against MMPs.