Does citrate lyase require ATP?

Reaction. ATP citrate lyase is responsible for catalyzing the conversion of citrate and Coenzyme A (CoA) to acetyl-CoA and oxaloacetate, driven by hydrolysis of ATP.

What does ATP citrate lyase do?

ATP citrate lyase (ACLY) is a key enzyme of de novo fatty acid synthesis responsible for generating cytosolic acetyl-CoA and oxaloacetate. Enhanced glucose and lipid metabolism is one of the most common properties of malignant cells.

Why does insulin activate citrate lyase?

263, 12677-12681]. Insulin also decreases the phosphorylation of peptide B but increases the phosphorylation of peptide A of ATP-citrate lyase (ATP-CL). These results suggest that the insulin-induced increase in ATP-CL phosphorylation at peptide A is due to a decrease in peptide B phosphorylation.

Is citrate synthase A lyase?

Citrate synthase (1CTS), originating in Sus scrofa, is a member of the Oxo Acid Lyase family (1). Citrate synthase acts in the first step of the citric acid cycle by catalyzing the condensation reaction between acetate (in the form of acetyl-CoA) and oxaloacetate to produce citrate, a six-carbon intermediate (7).

What is the function of the ATP citrate lyase?

ATP-citrate lyase (ACLY) catalyzes the ATP-dependent conversion of citrate and CoA to oxaloacetate and acetyl-CoA. It is one of the major sources of cytosolic acetyl-CoA, and is a central metabolic enzyme.

How is histone acetylation dependent on citrate lyase?

We have shown that histone acetylation in mammalian cells is dependent on adenosine triphosphate (ATP)-citrate lyase (ACL), the enzyme that converts glucose-derived citrate into acetyl-CoA.

What is the EC number of citrate lyase?

In the presence of ATP and CoA, citrate lyase catalyzes the cleavage of citrate to yield acetyl CoA, oxaloacetate, adenosine diphosphate (ADP), and orthophosphate (P i ): This enzyme was formerly given the EC number 4.1.3.8. The enzyme is cytosolic in plants and animals.

Which is an enzyme that converts citrate to acetyl CoA?

ATP citrate synthase (also ATP citrate lyase (ACLY)) is an enzyme that in animals represents an important step in fatty acid biosynthesis. By converting citrate to acetyl-CoA, the enzyme links carbohydrate metabolism, which yields citrate as an intermediate, with fatty acid biosynthesis, which consumes acetyl-CoA.