What is TK promoter?

thymidine kinase (TK) gene promoter contains a novel. element that can be functionally activated by TB. The transcriptional control elements of the TK gene. 319.

What is HSV tk gene?

– herpes simplex virus thymidine kinase (HSV-TK) gene. herpes simplex virus thymidine kinase (HSV-TK) gene. In the cell transduced with this gene, an expressed HSV-TK gene has effect on ganciclovir which is generally used as an anti-virus drug, and changes it to a toxic agent. So it induces the cell death (suicide).

What is the promoter sequence in DNA?

Promoter sequences are DNA sequences that define where transcription of a gene by RNA polymerase begins. Promoter sequences are typically located directly upstream or at the 5′ end of the transcription initiation site.

What is the normal function of a gene promoter?

In genetics, a promoter is a sequence of DNA to which proteins bind that initiate transcription of a single RNA from the DNA downstream of it. This RNA may encode a protein, or can have a function in and of itself, such as tRNA, mRNA, or rRNA.

How does HSV TK work?

HSV-1 is mainly transmitted by oral-to-oral contact to cause oral herpes infection, via contact with the HSV-1 virus in sores, saliva, and surfaces in or around the mouth. However, HSV-1 can also be transmitted to the genital area through oral-genital contact to cause genital herpes.

Is TK gene real?

Thymidine kinase is an enzyme, a phosphotransferase (a kinase): 2′-deoxythymidine kinase, ATP-thymidine 5′-phosphotransferase, EC 2.7. 1.21. It can be found in most living cells. It is present in two forms in mammalian cells, TK1 and TK2….

How does promoter work?

A promoter is a region of DNA where transcription of a gene is initiated. Promoters are a vital component of expression vectors because they control the binding of RNA polymerase to DNA. RNA polymerase transcribes DNA to mRNA which is ultimately translated into a functional protein.

Is the TATA box a promoter?

A TATA box is a DNA sequence that indicates where a genetic sequence can be read and decoded. It is a type of promoter sequence, which specifies to other molecules where transcription begins. Many eukaryotic genes have a conserved TATA box located 25-35 base pairs before the transcription start site of a gene. …

What is the main function of promoter?

“A promoter is the one who envisages an idea for setting up a particular business at a given place and carries out a variety of formalities required for starting a business.” A promoter is the one who envisages an idea for setting up a particular business at a given place and carries out a variety of formalities …

What is Tk disease?

TK2 deficiency basics. TK2d is an enzyme deficiency. It is a genetic disease that is defined by muscle weakness (myopathy), with effects like difficulty breathing, droopy or saggy eyelids, or trouble chewing and swallowing.

Which is stronger the TK promoter or the CMV promoter?

simplex virus (HSV) thymidine kinase (TK) promoter has been shown to be in general weaker than that of other widely used viral promoters, the strong CMV and SV40 promoters [33,34,38,42,43].

Which is the promoter sequence of herpes simplex virus?

Herpes simplex virus (HSV) thymidine kinase promoter. To obtain this annotated DNA sequence with restriction sites, please download SnapGene or the free SnapGene Viewer. Your time is valuable! × Please choose an application for opening sequence files.

Which is a mutant of the TK promoter?

Deletion mutants of the TK promoter were constructed and introduced into the Gateway system for ectopic expression of enhanced green fluorescent protein (eGFP), monomeric cherry (mCherry), and FFPs containing these FPs.

How is Tein expressed in herpes simplex virus?

tein expression in comparison with the commonly used, very strong CMV promoter, the herpes simplex virus thymidine kinase (TK) gene promoter, and mutants thereof were analyzed. Deletion mutants of the TK promoter were constructed and introduced into the Gateway system for ectopic expression of enhanced green fluorescent protein (eGFP), monomeric