What is ProLong diamond?

Description. ProLong Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides. It is formulated without DNA stain or with the blue DNA stain DAPI or with the deep red DNA stain SYTOX Deep Red.

How do you ProLong a diamond?

Just apply a drop to the sample, add a coverslip, cure, and image. ProLong Diamond mountant is a curing mountant that forms a near-perfect optical path (1.47 RI) and enables longer-term storage of your samples, while protecting them from photobleaching.

Does ProLong gold Harden?

ProLong™ Gold and Diamond Antifade Mountants are hard (curing) mountants that achieve an optimal refractive index of 1.46 after curing and can be used for long-term storage of slides. ProLong™ Gold Mountant can be conveniently stored at room temperature and provides a similar antifade protection for Alexa Fluor™ dyes.

How long does ProLong gold take to dry?

24 hours
Like the original ProLong® antifade reagent, ProLong® Gold reagent will cure within 24 hours, and the sample can be saved for months after mounting.

Can you use prolong Diamond on live cells?

No. ProLong, ProLong Gold, ProLong Diamond, and ProLong Glass reagents are intended for use on fixed or fixed/permeabilized samples. Only ProLong Live Antifade Reagent (Cat Nos. P36974 and P36975) is intended for use with live cells.

What is the RI of prolong Diamond mountant?

ProLong Diamond mountant will cure to an RI of 1.47. Additionally, this curing property means you do not need to use messy acrylic resins (i.e., finger nail polish), which can damage or ruin modern microscope objectives if it comes into contact with them. For Research Use Only.

Can you use prolong diamond for fluorescent dyes?

You can use ProLong Diamond mountant with almost any fluorescent dye or fluorescent protein (e.g., GFP, RFP, mCherry) to achieve the brightest signal and lowest background, for bright, high definition fluorescent images. Learn more about ProLong Antifade mountants.

How does prolong Diamond antifade mountant work?

ProLong™ Diamond mountant is designed to provide unparalleled antifade protection across the entire visual and IR spectrums. You can use ProLong™ Diamond mountant with almost any fluorescent dye or fluorescent protein (e.g., GFP, RFP, mCherry) to achieve the brightest signal and lowest background, for bright, high definition fluorescent images.