What is polyethylenimine used for?

Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery.

What is PEI Max?

Polyethylenimine “Max” (PEI MAX) is a powerful, trusted, and cost-effective reagent widely considered as a current gold standard for both in vitro and in vivo transfection. PEI MAX has a high density of protonatable amino groups, with amino nitrogen as every third atom.

How does polyethylenimine work?

Attachment promoter. Polyethyleneimines are used in the cell culture of weakly anchoring cells to increase attachment. PEI is a cationic polymer; the negatively charged outer surfaces of cells are attracted to dishes coated in PEI, facilitating stronger attachments between the cells and the plate.

Is PEI toxic to cells?

Despite high capacity for shuttling of genes in target cells (Fig. 2), the PEI itself can exert significant cellular toxicity so that the IC50 for LPEI and BPEI respectively were 74 mg and 37 mg (Fig. 3).

How is Polyethylenimine made?

Polyethylenimines (PEI) are low to high molecular weight compounds with the general formula -[CH2-CH2-NH2]-, made by ring opening polymerization of aziridine. These polymers are available as linear, partly branched or repetitively branched polymers (dendrimers).

Is Polyethylenimine organic?

Polyethylenimine (PEI), an organic branched or linear polyamine polymer, has been successfully used in the past for DNA complexation and transfection in vitro and in vivo into several cell lines and tissues.

How do you make Max PEI solution?

1. In 1L glass beaker, suspend 1g of PEI MAX 40K in 900 mL water. 2. Add PTFE-coated stir bar to 1L glass beaker and set stirring to produce small vortex.

What does PEI do in transfection?

DNA can be introduced into a host cell by transfection with polyethylenimine (PEI), a stable cationic polymer (Boussif et al., 1995). PEI condenses DNA into positively charged particles that bind to anionic cell surfaces.

What is polyethyleneimine alkoxylated?

Polyethyleneimine ethoxylate (PN-100) is water-soluble nonionic polymer and has various characteristics below. It is widely applicable.

Why is PEI toxic?

However, the safety of PEI is always a concern for its clinical translation. It has been reported that the positive charges of PEI could induce necrotic cell death and apoptosis and cause toxicity both in vitro and in vivo, which significantly limits its clinical usage.

Is PEI cytotoxic?

Polyethyleneimine (PEI) is a synthetic polymer commonly used as precursor base layer in polyelectrolyte multilayer films. These results suggest that PEI is potentially cytotoxic and may not be biocompatible enough in clinical applications using high molecular weight.

Is Polyethylenimine biodegradable?

Among the cationic polymers, polyethylenimine (PEI) is regarded to be one of the most successful and widely studied gene delivery polymers. In addition, HMW PEI, which is non-biodegradable, probably would give rise to the accumulation of cytotoxicity in vivo, so the long-term safety of HMW PEI is further problematic.

How is polyethylenimine used as a permeabilizer?

Poly (ethylenimine) is also an effective permeabilizer of the outer membrane of Gram-negative bacteria. Both linear and branched polyethylenimine have been used for CO 2 capture, frequently impregnated over porous materials.

What is the purpose of linear polyethylenimine ( PEI )?

Linear polyethylenimine (PEI) is a high-charge cationic polymer that readily binds highly anionic substrates. Industrially, linear PEI can improve the appearance of negatively charged dyes by modulating their properties and improving their adherence to surfaces.

Which is the repeating unit of polyethylenimine?

Polyethylenimine (PEI) or polyaziridine is a polymer with repeating unit composed of the amine group and two carbon aliphatic CH2CH2 spacer. Linear polyethyleneimines contain all secondary amines, in contrast to branched PEIs which contain primary, secondary and tertiary amino groups. Totally branched, dendrimeric forms were also reported.

What are the properties of polyethyleneimines at room temperature?

Properties. The linear PEIs are solids at room temperature while branched PEIs are liquids at all molecular weights. Linear polyethyleneimines are soluble in hot water, at low pH, in methanol, ethanol, or chloroform. They are insoluble in cold water, benzene, ethyl ether, and acetone. They have a melting point of 73–75 °C.