What is PCR explain briefly?

Polymerase chain reaction, or PCR, is a technique to make many copies of a specific DNA region in vitro (in a test tube rather than an organism). In PCR, the reaction is repeatedly cycled through a series of temperature changes, which allow many copies of the target region to be produced.

What are 4 important applications of PCR?

The polymerase chain reaction has been elaborated in many ways since its introduction and is now commonly used for a wide variety of applications including genotyping, cloning, mutation detection, sequencing, microarrays, forensics, and paternity testing.

What is the purpose of PCR?

The polymerase chain reaction (PCR) was originally developed in 1983 by the American biochemist Kary Mullis.

What are the steps in PCR protocol?

A standard PCR cycle includes three steps: denaturation (95 C), annealing (55 C), and elongation (65 C). Put each ingredients of a PCR reaction in with the step in the PCR cycle in which it is first used.

What is PCR primer?

According to the University of Wisconsin’s BioWeb website, a PCR primer is a short, synthetic oligonucleotide (usually between 18 to 25 bases long) used to amplify specific regions of DNA in a molecular biology technique known as polymerase chain reaction (PCR).

What is a PCR procedure?

Introduction. The polymerase chain reaction (PCR) is a procedure that mimics the cellular process of DNA replication using the machinery of heat-resistant bacteria in a cyclic manner, resulting in several million copies of a specific DNA sequence that can then be visualized through electrophoresis and staining with a dye.