What is Nano luciferase?

NanoLuc luciferase (NLuc) is the newest commercially available luciferase enzyme. NLuc is a 19.1 kDa luciferase enzyme that relies on the substrate furimazine to produce high intensity, glow-type luminescence. 4. Recently, NLuc was shown to exhibit several properties superior to traditional luciferases.

What is dual luciferase reporter assay?

The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing two reporter assays. After quantifying the firefly luminescence, this reaction is quenched, and the Renilla luciferase reaction is initiated simultaneously by adding Stop & Glo® Reagent to the same sample.

What is a luciferase assay?

A luciferase assay is used to determine if a protein can activate or repress the expression of a target gene. A separate DNA construct encodes the protein hypothesized to affect transcription. The scientist transfects a cell culture system, such as HEK 293T cells, with both constructs.

Is luciferase a plasmid?

Firefly and Renilla luciferase are less-than-ideal protein tags due to their large size. Luciferase-containing plasmids are commonly used to investigate the effect of regulatory elements, such as promoters, enhancers and untranslated regions, or the effect of mutations of these regulatory elements on gene expression.

How does Gaussia luciferase work?

Gaussia luciferase is a 20kDa protein from the marine copepod, Gaussia princeps. The bioluminescent enzyme is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity.

How does dual luciferase reporter assay work?

Where is luciferase used?

Luciferases are widely used in biotechnology, for microscopy and as reporter genes, for many of the same applications as fluorescent proteins. However, unlike fluorescent proteins, luciferases do not require an external light source, but do require addition of luciferin, the consumable substrate.

How does dual luciferase assay work?

The dual luciferase assay is performed by sequentially measuring the firefly and Renilla luciferase activities of the same sample, with the results expressed as the ratio of firefly to Renilla luciferase activity (Fluc/Rluc).

Is luciferin toxic to humans?

Luciferin Toxicity The small size of luciferin also makes it a poor antigen and immune responses to luciferin are unlikely. Luciferin is able to pass the blood brain barrier, the blood placenta barrier and the blood testis barrier, toxicity appears low.

Do humans have luciferin?

According to a study conducted in 2009 by Japanese researchers, human bioluminescence in visible light exists – it’s just too dim for our weak eyes to pick up on. “The human body literally glimmers,” the team from the Tohoku Institute of Technology wrote in their study published in PLOS One.

What is the role of luciferase?

Luciferase is an enzyme used for bioluminescence by various organisms in nature, most famously the firefly. If the protein is able to upregulate transcription of the target gene, the cells will express luciferase. If the protein downregulates transcription, the cells will express less luciferase than normal.

Are there any warranties of any kind for Promega?

PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. Patent Pending.

How does the Promega nano Glo luciferase assay system work?

Nano-Glo®Luciferase Assay System(a,b)provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc®luciferase. The reagent is prepared by mixing Nano-Glo®Luciferase Assay Substrate and Nano-Glo®Luciferase Assay Buffer and contains an integral lysis buffer to use directly on cells expressing NanoLuc

Where to buy Promega helix onsite stocking program?

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab’s needs.