What is heat inactivated serum?

The objective of heat inactivation is to destroy complement activity in the serum without affecting the growth-promoting characteristics of the product. Removal of complement activity from the serum is not required for most cell cultures, but may be necessary for cultures that are sensitive to the complement activity.

What is HyClone used for?

HyCloneTM Defined FBS is widely used by cell culturists who have a concern for viral contaminants and require an extensive biochemical profile. The final serum is assayed for over 50 components, including proteins, hormones, growth factors, metabolites, and nutrients important for cell culture.

What is the purpose of heat inactivated FBS?

A common treatment of FBS is heat-inactivation, where FBS is heated at 56°C for 30 minutes in a water bath with occasional shaking. The purpose is to inactivate whatever components of the complement system are present in the FBS [24], and other potential unknown inhibitors of cell growth.

What is HyClone FBS?

HyClone characterized fetal bovine serum (FBS) is produced using true pool technology, which minimizes bottle-to-bottle variability. This FBS product is filtered through three sequential 100 nm (0.1 µm) pore size-rated filters. Each lot of FBS is subjected to virus panel testing according to 9 CFR 113.53.

How do you prepare a heat inactivated serum?

How to heat inactivate serum:

1. Thaw the serum slowly (see above).
2. Prepare a water bath large enough to accommodate the number of bottles being treated.
3. Place bottles of thawed serum in the water bath.
4. Bottles should be agitated gently during the heat inactivation process to ensure uniform heating throughout the serum.

Why is serum heated?

Adult serum contains various immune factors, particularly serum complement, which may inhibit or destroy cells under certain conditions. Heating serum is intended to inactivate serum complement. Heat inactivation will increase precipitates and consequent turbidity in the serum.

How do you heat inactivate FBS?

Technical Tips 1 – Heat Inactivation of Fetal Bovine Serum

1. Thaw the serum slowly to 37°C and mix the contents of the bottle thoroughly.
2. Place the thawed bottle of serum into a 56°C water bath containing enough water to immerse the bottle to just above the level of the serum.
3. Begin timing for 30 minutes.

Why do we need to heat inactivated serum?

Is heat inactivation of serum necessary?

At one time, heat inactivation was considered necessary because of concerns over possible contaminants in serum. Things have changed. Today, many feel that exposing serum to heat degrades valuable biomolecules, such as growth factors, vitamins, and amino acids–and is no longer generally advisable.

Does FBS need to be heat inactivated?

In past years, Coriell used all heat-inactivated serum for its cell cultures to inactivate the complement protein found in newborn calf serum. Since we have changed to fetal bovine serum, we find that heat inactivation is not necessary for most cell lines.

Can I thaw FBS at 37 C?

Note: Thawing of fetal bovine serum at temperatures above 37 °C is not recommended. This process may degrade heat labile nutrients, thus compromising the integrity and performance of the product, and can cause increased precipitate formation. 5.

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