What is fish report in breast cancer?

Fluorescence in situ hybridization (FISH) is a test that “maps” the genetic material in a person’s cells. This test can be used to visualize specific genes or portions of genes. FISH testing is done on breast cancer tissue removed during biopsy to see if the cells have extra copies of the HER2 gene.

What is an example of human breast cell line?

1 MCF-7 human adenocarcinoma cells. MCF-7 is a human breast cancer cell line with estrogen, progesterone and glucocorticoid receptors [26].

What is MCF7 cell line?

MCF-7 is a human breast cancer cell line with estrogen, progesterone and glucocorticoid receptors [26]. It is derived from the pleural effusion of a 69-year-old Caucasian metastatic breast cancer (adenocarcinoma) in 1970 by Dr Soule of the Michigan Cancer Foundation, Detroit, MI [27].

How many breast cancer cell lines are there?

By analyzing the molecular features of 92 breast cancer cell lines as documented by different literatures, we categorize 84 cell lines into 5 groups to be consistent with breast tumor classification.

What is FISH report in cancer?

Fluorescence in situ hybridization (FISH) is increasingly being recognized as the most accurate and predictive test for HER2/neu gene amplification and response to therapy in breast cancer.

How do I read my FISH test results?

How your doctor interprets this test is as follows:

1. A result of 0 is negative.
2. A result of 1+ is also negative.
3. A result of 2+ is considered equivocal (uncertain).
4. A result of 3+ is positive.

How do I choose a cell line?

The Do’s and Dont’s of Choosing a Cell Line

1. Don’t Choose Simply Because It Is Used in the Literature.
2. Do Consider If It Fits Your Biological Model.
3. Do Consider the Experiments You Want to Perform.
4. Table 1: Features of commonly used cell lines.
5. Don’t Assume That All Cell Lines Have the Same Culturing Requirements.

What are T47D cells?

T47D and MCF7 are two human hormone-dependent breast cancer cell lines which are widely used as experimental models for in vitro and in vivo (tumor xenografts) breast cancer studies. Several proteins involved in cancer development were identified in these cell lines by proteomic analyses.

What type of cells are MCF7?

MCF-7 is a breast cancer cell line isolated in 1970 from a 69-year-old White woman. MCF-7 is the acronym of Michigan Cancer Foundation-7, referring to the institute in Detroit where the cell line was established in 1973 by Herbert Soule and co-workers.

What type of cells are MCF7 cells?

MCF-7 is an adherent, epithelial luminal cell line positive for estrogen and progesterone receptors. Although they are referred to as hormone-dependent, the minimal amount of steroid hormones present in serum is sufficient to ensure cell growth.

Why do we use cancer cell lines?

Cancer cells that keep dividing and growing over time, under certain conditions in a laboratory. Cancer cell lines are used in research to study the biology of cancer and to test cancer treatments.

How do I read my fish test results?

What do fish test results mean for breast cancer?

These HER2 receptors receive signals that stimulate the growth of breast cancer cells. The FISH test results will tell you that the cancer is either “positive” or “negative” (a result sometimes reported as “zero”) for HER2.

What are the different types of breast cancer cell lines?

List of cell lines Cell line Primary tumor Origin of cells Estrogen receptors Progesterone receptors 600MPE Invasive ductal carcinoma + – AMJ13 Invasive ductal carcinoma Primary Yes Yes AU565 Adenocarcinoma – – BT-20 Invasive ductal carcinoma Primary No No

Are there any crude models of breast cancer?

Breast cancer cell lines are crude models of breast cancer. Despite the considerable roles played by breast cancer cell lines in deciphering the mechanisms underlying tumor initiation and evolution, cell lines are still viewed as crude models of tumors and could not capture all tumor features and heterogeneity.

Can a fish test confirm a HER2 positive cancer?

In most cases, FISH testing can confirm whether the cancer is HER2-positive or HER2-negative. HER2 test results aren’t always accurate. If an initial FISH test is inconclusive or the results are considered borderline, your doctor may request an additional biopsy to test another sample.