Guidelines

What is czapek yeast extract agar?

What is czapek yeast extract agar?

Czapek Yeast Extract Agar is recommended for the cultivation and maintenance of Aspergillus brasiliensis (1). This medium supports the abundant growth of almost all saprophytic Aspergilli (2). Sucrose serves as the source of energy. Yeast extract provides essential amino acids, vitamins and other essential nutrients.

How do you make Czapek Dox Agar?

Czapek Dox Agar is a semisynthetic medium used for the general cultivation of fungi from water samples. Suspend 49.01 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

What is yeast extract used for in agar?

Yeast Extract Agar is used for the enumeration of microorganisms in potable and freshwater samples and is not intended for use in the diagnosis of disease or other conditions in humans. A nutrient agar corresponding to the standard formulation for the plate count of micro-organisms in water and dairy products.

What is CYA Agar?

Recommended for the isolation and cultivation of heat resistant filamentous fungi (molds) from foods.

How to prepare Czapek’s agar ( CZA ) Culture Media?

Preparation and Method of Use of Czapek’s Agar (CZA) Suspend 49.01 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.

How does Thermo Scientific use yeast extract agar?

Yeast Extract Agar (Dehydrated) Carry out plate counts of microorganisms in water with Thermo Scientific™ Oxoid™ Yeast Extract Agar (Dehydrated). Yeast Extract Agar is made to the formula described by Windle Taylor 1 for the plate count of microorganisms in water and is tested in accordance with ISO 1133:2014 2.

When to use czapek dox agar for isolation?

Czapek Dox Agar is recommended by APHA for isolation of Aspergillus, Penicillium, Paecilomyces and some other fungi with similar physiological requirements. Czapek Medium is recommended in Standard Methods for Examination of Water and Wastewater for use in the isolation of Aspergillus, Penicillium, and related fungi.