What does LY294002 inhibit?

LY294002 and wortmannin are chemical compounds that act as potent inhibitors of phosphoinositide 3-kinases (PI3Ks). Both of them are generally used to inhibit cell proliferation as cancer treatment by inhibiting the PI3K/protein kinase B (AKT) signaling pathway.

How does LY294002 inhibit PI3K?

The PI3K inhibitor LY294002 prevents induction of p53 by DNA damage. To explore the contribution of particular signal transduction cascades to the effect of serum growth factors on p53 induction, cells were exposed to CisPt in the presence of specific chemical inhibitors.

What does wortmannin do?

Wortmannin is a commonly used cell biology reagent and has been previously used to inhibit DNA repair, receptor-mediated endocytosis and cell proliferation [13].

What is an AKT inhibitor?

Maximal AKT activation requires phosphorylation by mTORC2. Moreover, AKT inhibits effector proteins via phosphorylation, including glycogen synthase kinase-3 (GSK3) β and forkhead family of transcription factors (FOXO). The signaling results in the regulation of cell proliferation, survival, and metabolism.

How do PI3K inhibitors work?

By inhibiting these enzymes, PI3K inhibitors cause cell death, inhibit the proliferation of malignant cells, and interfere with several signaling pathways.

What type of name is ly294002 a protein kinase inhibitor )?

LY294002 is a morpholine-containing chemical compound that is a potent inhibitor of numerous proteins, and a strong inhibitor of phosphoinositide 3-kinases (PI3Ks)….LY294002.

What does Akt stand for?

AKT

What is the full form of Akt?

Protein kinase B (PKB), also known as Akt, is the collective name of a set of three serine/threonine-specific protein kinases that play key roles in multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription, and cell migration.

What happens if PI3K is inhibited?

What inhibits PI3K?

CAL-101 (GS-1101, Idelalisib) CAL-101 (GS-1101, idelalisib) is an oral and specific inhibitor of the δ isoform of PI3K [122, 123]. It has been shown that idelalisib has therapeutic effects without inhibiting PI3K signaling essential for normal function of healthy cells [157, 158].

What is the function of Akt?

Akt stimulates cell growth and proliferation through mTORC1. Akt also increases VEGF secretion and mediates eNOS phosphorylation, vasorelaxation and angiogenesis. Akt can increase cellular metabolism through its downstream targets GSK3 and GLUT4.

How much is an Akt class?

The basics: Cost and equipment Access to the AKT GO platform costs $19.99 per month and, right now, you can snag a 7-day free trial.

Which is better wortmannin or LY294002 for pk59 cells?

These results indicate that LY294002, but not wortmannin, displays the opposite function to that expected in regulating the PI3K/AKT signaling pathway in PK59 cells. LY294002 induces upregulation of p-AKT in PC PK59 cells. (A) PK59 cells were treated with different dose of LY294002 for 24 h.

How is wortmannin used to treat pancreatic cancer?

LY294002 and wortmannin are chemical compounds that act as potent inhibitors of phosphoinositide 3-kinases (PI3Ks). Both of them are generally used to inhibit cell proliferation as cancer treatment by inhibiting the PI3K/protein kinase B (AKT) signaling pathway.

How is LY294002 used to treat pancreatic cancer?

LY294002 induces upregulation of p-AKT in PC PK59 cells. (A) PK59 cells were treated with different dose of LY294002 for 24 h. (B) PK59 cells were treated with 1 µg/ml of EGF for 30 min in the presence of 50 µM of LY294002 or 2 µM of wortmannin.

Where does wortmannin bind in the ATP binding site?

Wortmannin binds in the ATP binding site so that one face of wortmannin packs against the N-terminal lobe (residues 831, 879, 881, and 882) and the other face packs against the C-terminal lobe (residues 950, 953, 961, 963, and 964).