What does homogenate mean?

noun, plural: homogenates. Material that has been homogenized or obtained through homogenization. Supplement. For example, a liver homogenate is a liver tissue in which its cells have been mechanically disrupted, releasing the organelles and cytoplasm.

What is tissue homogenate?

A tissue homogenate (thom) is obtained through mechanical micro-disruption of fresh tissue and the cell membranes are mechanically permeabilized. Abbreviation: thom.

What does homogenised mean in biology?

Homogenization, process of reducing a substance, such as the fat globules in milk, to extremely small particles and distributing it uniformly throughout a fluid, such as milk.

What is the meaning of spectrometry?

Spectrometry is the measurement of the interactions between light and matter, and the reactions and measurements of radiation intensity and wavelength. In other words, spectrometry is a method of studying and measuring a specific spectrum, and it’s widely used for the spectroscopic analysis of sample materials.

What is Cell homogenization?

Cell biology Homogenization is a process that involves breaking apart cells – releasing organelles and cytoplasm. When the purpose is to extract organelles, it is frequently done in two steps; first using a blender to break the tissue up, and then with an ultrasonic or mechanical tissue disruptor.

How do you homogenize a tissue?

Cryohomogenization can be carried out using a supercooled mortar and pestle (classic approach), or the tissue can be homogenized by crushing it into a fine powder inside a clean plastic bag resting against a supercooled solid metal block (more recently developed and more efficient technique).

How is tissue homogenization done?

Homogenization: the process of breaking down tissue structure to form a suspension or emulsion of tissue solids, proteins and fluid. Homogenization techniques: mechanical, sonicated, bead-beating and enzymatic.

How do you homogenize brain tissue?

Homogenization can be done in homogenizer keeping your sample in ice bath to avoid heating. Three 15 second-pulses would be sufficient to disintegrate the tissue and please keep in ice bucket for 15 min for complete dissociation.

Why do we do homogenization?

First and foremost, the purpose of homogenization is to reduce the size of the particles making up a sample. In simple terms, it’s a bit like taking a boulder and smashing it into gravel, except, in this case, the gravel pieces would be all the same size and shape.

Who uses spectroscopy?

UV-VIS spectroscopy is commonly used by analytical chemists for the quantitative determination of different analytes, such as organic compounds, macromolecules, and metal ions. IR spectrophotometers use light wavelengths in the infrared range (700 – 15000 nm) of the electromagnetic spectrum.

Why is spectrometry important?

The amazing thing about spectrophotometry is that it can theoretically be used to measure any substance that absorbs light. For instance, spectrophotometry can quantify nucleic acids, proteins and bacterial density, but it also can measure bitterness compounds (IBUs, international bitterness units) in brewed beer!

What is the medical dictionary definition of homogenate?

Miller-Keane Encyclopedia and Dictionary of Medicine, Nursing, and Allied Health, Seventh Edition. © 2003 by Saunders, an imprint of Elsevier, Inc. All rights reserved. Tissue ground into a creamy consistency in which the cell structure is disintegrated (so-called cell-free). Compare: brei.

Which is the correct definition of the word homogenous?

[hōmoj′ənit] a tissue that is or has been made homogenous, as by grinding cells into a creamy consistency for laboratory studies. A homogenate usually lacks cell structure. Also called broken cell preparation.

What does it mean when something has been homogenized?

Something that has been homogenized. [ homogen (ize) + -ate .] American Heritage® Dictionary of the English Language, Fifth Edition. Copyright © 2016 by Houghton Mifflin Harcourt Publishing Company.

What’s the difference between brei and homogenate tissue?

Tissue ground into a creamy consistency in which the cell structure is disintegrated (so-called cell-free). Compare: brei. Tissue ground into a creamy consistency in which the cell structure is disintegrated (so-called cell free).