What are 293T cells?

The 293T cell line, originally referred as 293tsA1609neo, is a highly transfectable derivative of human embryonic kidney 293 cells, and contains the SV40 T-antigen. Age fetus Comments. This cell line is competent to replicate vectors carrying the SV40 region of replication.

What is difference between 293T and 293 ft?

293T cells and 293FT cells grow faster and are easier to transfect than HEK293 cells and result in higher titers of lentivirus. So most lentiviral protocols use 293FT cells to produce lentivirus.

Is HEK 293 Safe?

These organisms pose a moderate health hazard. Centers for Disease Control and Prevention (CDC) guidelines (pdf) require BSL-2 containment for work with HEK 293 cell lines.

Are HEK cells differentiated?

As adrenal cells are similar to neuronal cells, HEK293 cells indeed preferentially enter neuronal lineages upon spontaneous differentiation.

What can the HEK293 cell line be used for?

The HEK293 cell line (and its derivatives) are incredibly useful in research and can be used for a variety of applications across different fields of research. If you need a cell line that can produce large amounts of recombinant proteins, or if you’re a cell culture beginner looking for a low-maintenance cell line, HEK293 might be for you!

How did the 293T cell line get created?

The 293T cell line was created in the Michele Calos laboratory by transfection of a sub-line of adenovirus-immortalized human embryonic kidney cells with a gene encoding the SV40T-antigen and a neomycin resistance gene. The cell line is able to replicate vectors carrying the SV40 origin of replication.

How are adenoviral genes used in HEK293T cells?

Incorporating the adenoviral genes into the HEK cell genome resulted in the cells becoming very efficient at producing high amounts of recombinant proteins from plasmid vectors carrying the CMV promoter region. So, are HEK293T cells the same thing? Not exactly.

What kind of cell line is Stratagene ad-293?

INTRODUCTION Stratagene’s AD-293 cell line is a derivative of the commonly used HEK293 cell line, with improved cell adherence and plaque formation properties.