Is FITC the same as fluorescein?

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm and 519 nm, giving it a green color.

What is the excitation wavelength of fluorescein?

475-490 nm
The disodium salt of fluorescein is the most frequently used fluorophore in assessing tear turnover and corneal permeability. 9 In this application, the excitation wavelength was 475-490 nm and emission wavelength was 510-520 nm with peak fluorescence at pH 7.4. For Laboratory Use Only.

What wavelength is fluorescence?

In general, fluorescence investigations are conducted with radiation having wavelengths ranging from the ultraviolet to the visible regions of the electromagnetic spectrum (250 to 700 nanometers).

Is FITC a fluorescent label?

Fluorescein derivatives are the most common fluorescent reagents for biological research because of their high absorptivity, excellent fluorescence quantum yield, and good water solubility. A relatively broad fluorescence emission spectrum. Fluorescence quenching on conjugation to biopolymers.

What is FITC in flow cytometry?

Fluorescein isothiocyanate ( FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. First described in 1942, FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure.

What does FITC mean?

This page is all about the meaning, abbreviation and acronym of FITC explaining the definition or meaning and giving useful information of similar terms. FITC Stands For : Financial Institutions Training Centre | Fishery Industrial Technology Center.

What does FITC bind to?

BioLegend’s FITC Annexin V Apoptosis Detection Kit with 7-AAD has been specifically designed for the identification of apoptotic and necrotic cells. Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner.