How to dissolve sphingosine1 phosphate?

Sparingly soluble in 1:1 ethanol:water. Addition of small amounts of acetic acid may help dissolve this product. Soluble in warm methanol. For delivery of sphingosine-1-phosphate to cells, prepare a 125μM stock in 4mg/ml BSA as follows: Dissolve sphingosine-1-phosphate (S-1-P) in methanol (0.5 mg/ml).

What does sphingosine-1-phosphate do?

Introduction. Sphingosine 1-phosphate (SPP) is a bioactive sphingolipid metabolite that functions both as an intracellular second messenger and extracellularly as a ligand for specific G-protein-coupled receptors. These dual roles make SPP a unique and pivotal lipid in several signal transduction pathways.

Does sphingosine have a phosphate group?

This leads to the formation of sphingosine-1-phosphate, a potent signaling lipid. Sphingolipid metabolites, such as ceramides, sphingosine and sphingosine-1-phosphate, are lipid signaling molecules involved in diverse cellular processes.

What does S1P stand for?

Sphingosine 1-phosphate
Sphingosine 1-phosphate (S1P) is a bioactive lipid that is characterized by a peculiar mechanism of action.

Is sphingosine an alcohol?

Sphingosine (2-amino-4-octadecene-1,3-diol) is an amino alcohol with an 18-carbon unsaturated alkyl chain, forming a primary component of other sphingolipids.

What produces S1P?

S1P is produced in cells by two sphingosine kinase isoenzymes, SphK1 and SphK2. Many cells secrete S1P, which can then act in an autocrine or paracrine manner. Most of the known actions of S1P are mediated by a family of five specific G protein-coupled receptors.

What is a sphingosine MCAT?

Sphingolipids are a type of lipid that are made up of a sphingosine backbone attached to one fatty acid tail through an amide linkage. Additionally, sphingolipids contain a variable polar head group. Sphingolipids are amphipathic molecules, and function to maintain structural integrity of our cell membranes.

What do S1P receptors do?

Sphingosine-1-phosphate (S1P) is a signaling lipid that regulates many cellular processes in mammals. One well-studied role of S1P signaling is to modulate T- cell trafficking, which has a major impact on adaptive immunity. Compounds that target S1P signaling pathways are of interest for immune system modulation.

What is sphingosine made of?

Is sphingosine soluble in water?

Properties

Is sphingosine saturated?

The fully-saturated analogue of sphingosine, dihydrosphingosine, is also almost invariably present along with sphingosine. According to this nomenclature, sphingosine is 4-sphingenine (the prefix 4 indicates the position of the double bond and phytosphingosine is termed 4-hydroxysphinganine.

What is the role of sphingosine 1 phosphate?

Sphingosine 1-phosphate is a phosphosphingolipid that consists of sphingosine having a phospho group attached at position 1 It has a role as a vasodilator agent, a sphingosine-1-phosphate receptor agonist, a signalling molecule, a T-cell proliferation inhibitor and a mouse metabolite.

What kind of lipid is D-erythro-sphingosine-1-phosphate?

D-erythro-sphingosine-1-phosphate. Sphingosine-1-phosphate (S1P), a zwitterionic sphingolipid, is an important cellular metabolite and signalling lipid in animal cells. It has also been found in insects, yeasts and plants. You can find additional information on S1P at the following link:

How is S1P dephosphorylated to sphingosine in the blood?

S1P can be dephosphorylated to sphingosine by sphingosine phosphatases and can be irreversibly degraded by an enzyme, sphingosine phosphate lyase. S1P is a blood borne lipid mediator, in particular in association with lipoproteins such as high density lipoprotein (HDL).

How are phosphosphingolipids different from other sphingolipids?

Phosphosphingolipids are sphingolipids with a structure based on a sphingoid base that is attached to a phosphate head group. They differ from phosphonospingolipids which have a phosphonate head group.