Can I Vortex streptavidin beads?

Add 1mL of Binding/Wash Buffer to the tube. Invert the tube several times or vortex gently to mix. Collect the beads with a magnetic stand, then remove and discard the supernatant. Note: Do not allow beads to dry.

What is streptavidin beads?

Streptavidin Magnetic Beads are 1 µm superparamagnetic particles covalently coupled to a highly pure form of streptavidin. The beads can be used to capture biotin labeled substrates including antigens, antibodies and nucleic acids.

Can you reuse streptavidin beads?

For most applications involving dissociation of biotinylated molecules from streptavidin it is not possible to reuse the beads. The streptavidin-biotin bond is one of the strongest biological bonds known, and the conditions necessary to break this bond also destroy the streptavidin.

How do you clean streptavidin beads?

General Immobilization Protocol Place the tube in a magnet for 2-3 mins and discard the supernatant. Wash the coated beads 3-4 times in washing buffer. Resuspend to desired concentration in a suitable buffer for your downstream use.

What can streptavidin magnetic beads be used for?

These streptavidin magnetic beads are validated and optimized for use with high-throughput magnetic platforms, such as the Thermo Scientific KingFisher 96 and KingFisher Flex Instruments, but the beads also enable premium performance for simple benchtop applications using an appropriate magnetic stand.

How are Thermo Scientific Pierce magnetic beads used?

Thermo Scientific Pierce Streptavidin Magnetic Beads accelerate throughput for automated magnetic purification of biotinylated molecules. These streptavidin magnetic beads are validated and optimized for use with high-throughput magnetic platforms, such as the Thermo Scientific KingFisher 96 and KingFisher Flex Instruments,…

What can streptavidin agarose beads be used for?

Streptavidin is a tetrameric biotin-binding protein. The agarose beads have physical and chemical properties that enable them to be used in a variety of batch- or column-type affinity procedures.

What is the isoelectric point of Pierce streptavidin?

The native protein from Streptomyces avidinii is carbohydrate-free, has an acidic pI of 5.5 and a mass of 75 kDa. The recombinant protein used to manufacture Thermo Scientific Pierce Streptavidin Resins has a near-neutral isoelectric point and a mass of 53kDa.