Can Giardia affect kidneys?

We present a case of acute interstitial nephritis in association with Giardia infection in a 54-year-old woman who developed an impairment of renal function after a prolonged period of slight fever and diarrhea.

What stain is used for Giardia?

The staining methods which are used are the Iron-hematoxylin stain, the Trichrome stain and the Giemsa stain [5]. Direct Immunofluorescence – the Giardia cysts appear as oval, apple – green fluorescing forms which are 11-14 µm in diameter [10].

Is Giardia lamblia gram positive or negative?

It has a broad spectrum of antimicrobial activity, including gram positive and gram negative aerobic bacteria. In Giardia, the drug is reduced and activated in the trophozoite, possibly via NADH oxidase. The toxicity of the metabolites is mediated via DNA binding and destruction.

Is there a way to stain Giardia lambliacysts?

The Giardia lambliacysts could not be stained by this method. 20 stool samples were used as negative controls. Conclusion We developed the methylene blue staining for demonstrating the trophozoite of Giardia, which is a very simple permanent staining method. The slides can be kept for a permanent record.

Are there any positive trophozoites for Giardia lamblia?

All the 15 known Giardia lamblia trophozoite positive samples were also found to be positive by the Methylene blue staining. The Giardia lamblia cysts could not be stained by this method. 20 stool samples were used as negative controls.

How did Giardia lamblia get its name?

Giardiasis, also known as “beaver fever”, is caused by an infection with the gastrointestinal parasite, Giardia lamblia. This parasite was first discovered by the “Father of the Microscope”, Antoine van Leeuwenhoek, and later described by and named after Alfred Mathieu Giard and Vilém Dušan Lambl.

How to stain Giardia trophozoite with trichrome stain?

Place slides in 70% ethanol for 5 minutes. Drain excess liquid. Place slides in a second jar of 70% ethanol for 3 minutes. Place slides in Wheatley Trichrome stain for 10 minutes. Place slides in acid ethanol for 1-3 seconds.