What is the dependent variable in the enzyme catalysis lab?

The dependent variable is the rate of the reaction. The independent variable is the concentration of enzyme, the concentration of the substrate, the temperature, and the addition of an inhibitor. Introduction: Enzymes are proteins that carry out chemical reactions.

What conclusion can you draw about the effect of enzyme concentration on enzyme activity?

By increasing the enzyme concentration, the maximum reaction rate greatly increases. Conclusions: The rate of a chemical reaction increases as the substrate concentration increases. Enzymes can greatly speed up the rate of a reaction. However, enzymes become saturated when the substrate concentration is high.

What conclusion can you draw to explain the effect of temperature on enzyme activity?

Temperature affects the reaction rate of enzymes, as do pH, substrate concentration and enzyme concentration. At low temperatures, enzymes have low activity. As the temperature rises the rate of reaction increases, usually 2-fold for every 10 degree Celsius rise.

What is the hypothesis of the enzyme lab?

Hypothesis statement: If the enzyme catalase is in low concentration, then it will work better than if it were in high concentration.

What factors affect enzyme activity lab?

Several factors affect the rate at which enzymatic reactions proceed – temperature, pH, enzyme concentration, substrate concentration, and the presence of any inhibitors or activators.

What happens when there is more enzymes than substrate?

This is because the enzyme can’t work any faster even though there is plenty of substrate available. So when the amount of available substrate exceeds the amount of enzymes, then no more substrate can be broken down. The enzyme concentration is the limiting factor slowing the reaction.

How does temperature affect enzyme activity?

As with many chemical reactions, the rate of an enzyme-catalysed reaction increases as the temperature increases. However, at high temperatures the rate decreases again because the enzyme becomes denatured and can no longer function. As the temperature increases so does the rate of enzyme activity. …

How does temperature affect catalase enzyme activity?

Effects of Temperature As the temperature increases toward the optimum point, hydrogen bonds loosen, making it easier for catalase to act on hydrogen peroxide molecules. If the temperature increases beyond the optimum point, the enzyme denatures, and its structure is disrupted.

What does an enzyme inhibitor do?

Enzyme inhibitors are compounds which modify the catalytic properties of the enzyme and, therefore, slow down the reaction rate, or in some cases, even stop the catalysis. Such inhibitors work by blocking or distorting the active site.

What is enzyme and its function?

An enzyme is a type of protein found within a cell. Enzymes create chemical reactions in the body. They actually speed up the rate of a chemical reaction to help support life. The enzymes in your body help to perform very important tasks. An enzyme’s shape is tied to its function.

What is an enzyme example?

Examples of specific enzymes There are thousands of enzymes in the human body, here are just a few examples: Lipases – a group of enzymes that help digest fats in the gut. Amylase – helps change starches into sugars. Maltase – also found in saliva; breaks the sugar maltose into glucose.

Which is the independent variable in enzyme catalysis?

The pH of the solution was the independent variable. 10 mL of 1% hydrogen peroxide solution was added to each beaker. 10 mL of the correct buffer solution was added to the first corresponding beaker. 10 mL of the extracted catalase was added to the same beaker, which initiated the reaction. The reaction was timed for 180 seconds, or two minutes.

What are the steps of enzyme catalysis lab report?

1. Pour 5ml of substrate, 0ml of enzyme (no enzyme for negative control), and .5ml of water into test tube A1. 2. Seal test tube A1 with a stopper immediately after the enzyme is added. 3. Record the reaction rate using the Logger Pro software. 4. Repeat steps 1-3 using the new measurements as described in table 1. Table 1

How are enzymes efficient catalysts for biochemical reactions?

Enzyme lab Conclusion – Conclusion Enzymes are very… This preview shows page 1 – 2 out of 4 pages. Conclusion Enzymes are very efficient catalysts for biochemical reactions. They speed up reactions by providing an alternative pathway of lower activation energy.

How to measure the effect of temperature on enzyme catalysed reaction?

Set up the water baths at 10°C, 20°C, 30°C, 40°C and 50°C and put a beaker of lipase, containing a 2 cm 3 syringe into each water bath. Label a test tube with the temperature to be investigated. Add 5 drops of phenolphthalein to the test tube. Measure out 5 cm 3 of milk using a measuring cylinder and add this to the test tube.