What is serological pipetting?

The serological pipette is a nearly ubiquitous laboratory instrument used for transferring milliliter volumes of liquid. Serological pipettes typically have gradations along their sides for measuring the amount of liquid being aspirated or dispensed.

When should you use a serological pipette?

The best time to use serological pipette is when transferring liquids between receptacles, mixing chemical solutions, or laying reagents that are composed of different densities. Each of these procedures requires a high attention to detail when it comes to the aspirating and dispensing of your solution.

How do you do Micropipetting techniques?

Steps to follow when using a micropipette

1. Select the volume.
2. Set the tip.
3. Press and hold the plunger at the first stop.
4. Place the tip in the liquid.
5. Slowly release the plunger.
6. Pause for a second and then move the tip.
7. Insert the tip into the delivery vessel.
8. Press the plunger to the second stop.

What are the two types of pipettes?

What are the Different Types of Pipettes Used in Dentistry?

• Disposable Pipette. The disposable pipette is the most basic version of this tool.
• Graduated Pipette.
• Single-Channel Pipette.
• Multichannel Pipette.
• Repeat Dispensing Pipette.

How accurate is a serological pipet?

The acceptance level for product is ≤ 0.125 EU/mL or ≤ 5 EU/device. Serological pipettes of 1 and 2 mL are accurate to +/-3% at full volume in compliance with ASTM E934, “Standard Specification for Serological Pipettes, Disposable Plastic” and ISO 12771, “Plastics Laboratory Ware – Disposable Serological Pipettes”.

What are the different sizes of micropipettes?

We use three different sizes of micropipettes in the laboratory, the P20, P200 and P1000.

What do you need to know about Serological pipettes?

Serological pipettes are typically either plastic, sterile, and disposable or glass, sterilizable and reusable. All serological pipettes require the use of a pipette dispenser for the transfer of liquids. The pipette dispenser negates the need for a researcher to perform the ancient technique of mouth pipetting.

What are the steps to improve pipetting accuracy?

Step 1: The tip is immersed to the correct depth and correctly held vertically. Step 2: Inaccuracy doubles when immersing the tip too deeply. Step 3: Inaccuracy increases three to five times by immersing too deeply while holding the pipette at a 30-40° angle.

What’s the proper depth for a pipette before aspiration?

Immerse the tip to the proper depth during aspiration Before aspirating, immerse the tip adequately below the meniscus. Large volume pipettes (1-5 mL) should be immersed to 5-6 mm, while smaller volume pipettes should be immersed to 2-3 mm. Too little immersion, particularly with large volume pipettes, can lead to air aspiration.

What’s the best way to set a pipette?

Set your pipette to the desired volume within the pipette’s range. It’s a good idea to “prime” the pipette tip a few times by pipetting up and down with water before you take your first measurement.