What is lost prime in hplc?

If your mobile phase A bottle is empty or the tubing is not correctly emerged in the mobile phase, that will introduced air in to the system and it can lead to “lost prime”. HPLC software can detect extensive pressure variations or you can add a minimum back pressure at which point the method/pump will shut down.

What is wet prime in HPLC?

Wet prime pumps (often called ‘self-primers’) require the casing to be filled with water prior to the first start-up only. After that, the liquid will always remain in the pump volute, so the suction hose can run dry without damaging the pump or seals.

What is the troubleshooting in HPLC?

Pumping system problems are usually easy to spot and correct. Some of the more common symptoms are erratic retention times, noisy baselines, or spikes in the chromatogram. Leaks at pump fittings or seals will result in poor chromatography. Buffer salts should be flushed from the system daily with fresh deionized water.

What causes pressure drop in HPLC?

There can be several reasons for the pressure drops you observe: air could still be present in the system after your recent repair (i.e. between the degasser and the column). You could flush the system again with isopropanol on all channels to get rid of any residual air.

What are the problems with the HPLC system?

1. Column Life-Time 2. Variable Retention Times 3. Drifting Retention Times 4. Column-to-Column and Batch-to-Batch Reproducibility 5. Sample Preparation Problems 6. Sources of Peak Tailing 7. Normal-Phase Chromatography 8. System Volume, Dead Volume, Dwell Volume 9. Transfer of Gradient Methods 10. Clogged System 11. Column Plate-Count 12.

How to troubleshoot a HPLC with high back pressure?

Remove column from the system and turn on pump. If high back pressure still appears, then the blockage is in the system: If the pressure is normal, there is a problem with the column: See section High back pressure. See sections No pressure reading, no flow and No pressure reading, flow is normal

What causes pressure fluctuation in a HPLC line?

Improper Priming of the System — Failure to flush ALL of the lines with freshly degassed mobile phase, before use (every day), will often result in all kinds of instability problems until all of the old gas-filled mobile phase has bee purged from the system. *This could take many column volumes of liquid.

What causes lost Prime in a chromatography system?

If your mobile phase A bottle is empty or the tubing is not correctly emerged in the mobile phase, that will introduced air in to the system and it can lead to “lost prime”. HPLC software can detect extensive pressure variations or you can add a minimum back pressure at which point the method/pump will shut down.