What is electrophoresis for thalassemia?

Hemoglobin electrophoresis is a blood test that measures different types of a protein called hemoglobin in your red blood cells. It’s sometimes called “hemoglobin evaluation” or “sickle cell screen.”

What are the Hb electrophoresis in thalassemia major?

The distinguishing finding in beta thalassemia is a hemoglobin electrophoresis with the finding of elevated Hgb A2 and F. Both will be increased in beta thalassemia trait without iron deficiency, and will be normal or decreased in alpha thalassemia and isolated iron deficiency anemia.

What is normal pattern of hemoglobin electrophoresis?

Normal hemoglobin electrophoresis pattern: When hemoglobin electrophoresis reveals a normal pattern, i.e., HbF is <1%, HbA2 is <3.5% and the remainder is HbA, check the CBC values. If the MCV and MCH are not low, the case can be signed out as normal hemoglobin electrophoresis.

What is normal range of HbA?

Results and interpretation: The expected normal range for HbA2 is between 1.7% and 3.2% in normal subjects, while in β-thalassaemia carriers when it is between 4.0% and 7%. HbA2 values are considered borderline when between 3.2% and 3.8%.

What is electrophoresis used for?

Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size. In gel electrophoresis, the molecules to be separated are pushed by an electrical field through a gel that contains small pores.

How is thalassemia detected?

If your doctor suspects your child has thalassemia, he or she can confirm a diagnosis with blood tests. Blood tests can reveal the number of red blood cells and abnormalities in size, shape or color. Blood tests can also be used for DNA analysis to look for mutated genes.

Why is HbF high in thalassemia?

These data suggest that the high HbF levels in HbE/beta thalassemia, and other beta thalassemia syndromes, result from increased erythropoietin levels leading to bone marrow expansion, and possibly increased F-cell production, combined with ineffective erythropoiesis giving a survival advantage to F cells.

Why is Hb electrophoresis test done?

Hemoglobin electrophoresis measures hemoglobin levels and looks for abnormal types of hemoglobin. It’s most often used to help diagnose anemia, sickle cell disease, and other hemoglobin disorders.

What is hemoglobin electrophoresis used for?

What is HbF pregnancy?

Serial maternal serum hemoglobin F (HbF) levels were measured by radioimmunoassay in 23 normal pregnancies. The maternal HbF fraction remains within the normal range during the first trimester (mean + 2 S.D. = 0.15 + 0.13). After 15 weeks gestation, maternal HbF levels rise in some, but not all, pregnancies.

What is HbF blood test?

This test checks the amount of fetal hemoglobin (HbF) in the blood. Fetal hemoglobin is 1 of many types of hemoglobin. A baby growing in the womb has high levels of HbF. The level of HbF usually drops to tiny amounts about 6 months after birth.

What blood tests are done to detect thalassemia?

Abnormally shaped red blood cells are a sign of thalassemia. The lab technician may also perform a test known as hemoglobin electrophoresis . This test separates out the different molecules in the red blood cells, allowing them to identify the abnormal type.

What is the CPT code for hemoglobin electrophoresis?

Hemoglobin Electrophoresis. Test Code. 275. CPT Code(s) 83020. Preferred Requirement(s) 3mL (2mL) – Lavender ( EDTA ) Top Tube.

What is an electrophoresis test?

Definition Hemoglobin electrophoresis (also called Hgb electrophoresis), is a test that measures the different types of hemoglobin in the blood. The method used is called electrophoresis, a process that causes movement of particles in an electric field, resulting in formation of “bands” that separate toward one end or the other in the field.