What is Cary-Blair media used for?

Remel Cary Blair Transport Medium is a semisolid medium recommended for use in the transportation and preservation of clinical specimens, primarily stool and rectal swabs. This medium has a low nutrient content to prevent replication of organisms while maintaining viability.

What is a Cary-Blair container?

Cary-Blair with Indicator is an isotonic and nonnutritive medium, which does not contain a fermentable carbohydrate. Sodium thioglycollate is added to impede oxidation. Disodium phosphate is incorporated in the medium to act as a buffering agent, which prevents overgrowth of Escherichia coli and Enterobacter species.

What makes Cary-Blair medium a good transport medium for Vibrio species?

Alkaline pH of the medium minimizes bacterial destruction due to the formation of acid. Since this transport medium has a high pH (8.4), the viability of Vibrio cultures can be maintained for a longer duration and it is considered as the medium of choice for transport and preservation of Vibrio cholerae.

Should Cary-Blair be refrigerated?

Specimens in Cary-Blair transport media are acceptable up to 3 days if stored at 4C. However, performing culture from transport media within 24 hours assures optimal recovery of pathogens. Viral Culture If collecting sample with a swab use cotton tip with plastic or aluminum shaft or polyester swabs.

How do you use Cary Blair?

Preparation of Cary-Blair transport medium:

1. Suspend 12.6 grams in 991 ml distilled water.
2. Heat to boiling to dissolve the medium completely.
3. Cool to 50°C and aseptically add 9 ml of 1% aqueous calcium chloride solution.
4. Adjust pH to 8.4 if necessary.

What is Amies gel?

Clinical Microbiology Simplify the transportation of throat, vaginal, wound and skin swabs with Thermo Scientific™ Oxoid™ Amies Agar Gel Transport Swabs. Amies Agar Gel Transport Swabs come with soft rayon tips, which are inert and non-toxic to microorganisms.

When should you do a stool culture?

A stool culture may be ordered when someone has signs and symptoms of an infection of the digestive tract, such as: Diarrhea that lasts more than a few days and may contain blood and/or mucus. Abdominal pain and cramping. Nausea, vomiting.

Why do we need to transport the throat swab using transport culture medium?

Transport medium aims to preserve a specimen and minimize bacterial overgrowth from the time of collection to the processing of the specimen. Depending on the type of organisms suspected in the sample, transport media may vary.

Can I keep a stool sample overnight?

Stool samples should be delivered to the laboratory as soon as possible. If you can’t hand the stool sample in immediately you should store it in a fridge (but for no longer than 24 hours). Place the container in a sealed plastic bag first.

What is a para Pak?

Para-Pak CS Stool Transport Vials Containing C & S Medium. Parapak C&S (culture and sensitivity) is a system for collection, transportation and preservation of bacterial enteric pathogen, when maximum sensitivity is required from a stool culture procedure.

What is Amies medium?

Amies liquid medium is a nonnutritive balanced salt solution containing inorganic phosphates to provide buffering capability, sodium chloride, potassium chloride, calcium chloride and magnesium chloride to provide essential ions that help maintain osmotic balance.

What is a charcoal swab used for?

These swabs are used to test for thrush and/or BV.

What is the purpose of Cary Blair transport medium?

CARY BLAIR TRANSPORT MEDIUM. INTENDED USE. Remel Cary Blair Transport Medium is a semisolid medium recommended for use in the transportation and preservation of clinical specimens, primarily stool and rectal swabs. SUMMARY AND EXPLANATION.

How do you dispense Cary Blair Culture Media?

Dispense the medium in 7ml amounts in screw-cap bottles of 9 ml capacity (large size Bijou bottles). Sterilize by steaming with caps loosened (do not autoclave) at 100°C for 15 minutes.

What’s the shelf life of Cary Blair medium?

Store in a cool dark place with the bottle tops screwed tightly. pH of medium: This should be within the range pH 8.3 -8.5 at room temperatures. Shelf life: Cary-Blair transport medium is quite stable if stored in tightly sealed containers in a cool dark place so that the medium does not dry out.

Why is Cary Blair with indicator stored at room temperature?

Save valuable storage space with a transport that is stored in its original container at room temperature. Cary-Blair with Indicator is an isotonic and nonnutritive medium, which does not contain a fermentable carbohydrate. Sodium thioglycollate is added to impede oxidation.