What is Bicine used for?

It is commonly used in protein crystallization, in the study of enzymatic reactions, and for electrophoresis. It is used in thin layer ion exchange chromatography for protein resolution, as well as a multiphasic buffer system for SDS-PAGE of proteins. It is soluble in water at 0°C up to 1.1 M.

Is Bicine an acid or base?

N,N-bis(2-hydroxyethyl)glycine is a bicine that is a Good’s buffer substance, pKa = 8.35 at 20 ℃. It is a conjugate acid of a [bis(2-hydroxyethyl)amino]acetate. It is a tautomer of a [bis(2-hydroxyethyl)ammonio]acetate.

Is bicine an acid?

Bicine is an organic compound used as a buffering agent….Bicine.

How do you make bicine buffer?

Bicine (1 M, pH 8.26) Preparation and Recipe

1. Prepare 800 mL of dH2O in a suitable container.
2. Add 163.17 g of Bicine to the solution.
3. Adjust solution to desired pH with 10N NaOH.
4. Add dH2O until volume is 1 L.

What is Hepes buffer used for?

HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture.

What is MES buffer used for?

MES is often used in buffered culture media for bacteria, yeast, and mammalian cells. It is toxic towards plants at high concentrations, but can be used in culture media at lower concentrations for plants. Due to low ionic mobility, MES is regarded as an excellent buffer for use in capillary electrochromatography.

Are buffers weak acids?

A buffer is simply a mixture of a weak acid and its conjugate base or a weak base and its conjugate acid. Buffers work by reacting with any added acid or base to control the pH. Because that proton is locked up in the ammonium ion, it proton does not serve to significantly increase the pH of the solution.

Is HEPES toxic?

HEPES buffered media are resistant to rapid, drastic pH changes, but will not prevent pH shifts entirely. Since high levels of HEPES can cause cytotoxicity, concentrations of this organic buffer should be reduced if toxicity is apparent for a specific cell line or primary cell culture.

Is HEPES better than Tris?

If you want to maintain the pH at 7.5, HEPES is better than Tris. If you want to maintain it at 8.0, Tris is better. There are other considerations in choosing the buffer to use, however. Some enzymes may show a higher activity level in one buffer over another.

Is MES toxic?

Contact with this buffer is hazardous; skin or eye exposure should be cleaned well with water and medical aid should be sought in the case of eye exposure, swallowing or inhalation of dust. It also emits toxic fumes upon combustion, including carbon monoxide, nitrogen oxide and sulfur oxides.

What is MES monohydrate?

MES monohydrate is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. MES is often used in buffered culture media for bacteria, yeast, and mammalian cells.

Why can’t a buffer have a strong acid?

Buffers cannot be made from a strong acid (or strong base) and its conjugate. This is because they ionize completely! It is important to be able to recognize buffer solutions! Or, we could start with 1.0 M sodium acetate and add 0.5 M hydrochloric acid (this makes 0.5 M acetic acid).

What does bicine do to an amine system?

Bicine is a very corrosive degradation product produced in certain DEA and MDEA systems. Amine system operators must take steps to minimize O2 and SO2 incursion into the amine system and will need to monitor Heat Stable Salts and Amino Acids (Bicine) concentrations in the amine.

How is Bicine used as a buffering agent?

Bicine. Bicine is an organic compound used as a buffering agent. It is one of Good’s buffers and has a pKa of 8.35 at 20 °C. It is prepared by the reaction of glycine with ethylene oxide, followed by hydrolysis of the resultant lactone. Bicine is a contaminant in amine systems used for gas sweetening.

What can Bicine be used for in biological research?

Bicine is a general purpose buffer for biological research. Useful pH range is 7. 6 – 9. Its applications include: tissue culture, phosphorylation and photophosphorylation, fixative transmission electron microscopy, protein synthesis and preventing binding to non-receptor materials. It is a degradation product of alkanolamine…

Where is Bicine found in a natural gas plant?

Bicine, an amino acid, has been found in numerous amine gas treating systems including tail gas treating units, certain refinery services, and some natural gas processing plants.