What is a pGEX vector?

pGEX-2TK is designed to allow the detection of expressed proteins by directly labeling the tagged products in vitro. This vector contains the recognition sequence for the catalytic subunit of cAMP-dependent protein kinase obtained from heart muscle.

What is pGEX plasmid?

Plasmid: pGEX-1 Expression vector permitting production of a fusion protein. (ATCC staff) The glutathione S-transferase (GST) fusion protein can be purified by glutathione affinity chromatography, and the desired polypeptide released from the fusion product by thrombin.

What is pGEX 2T?

pGEX-2T. Description: pGEX-series GST fusion vector with Ptac promoter, lacI repressor, thrombin cleavage and kinase site ORFs; amp resistance; restriction enzyme cloning.

What is gst vector?

The GST tag GST is a 211 amino acid protein (26 kDa) whose DNA sequence is frequently integrated into expression vectors for production of recombinant proteins.

Which is the correct sequence for pgex-4t-1?

Bacterial vector for expressing fusion proteins with a thrombin site. For other reading frames, use pGEX‑4T-2or pGEX‑4T-3. To see this sequence with restriction sites, features, and translations, please download SnapGeneor the free SnapGene Viewer.

Which is the bacterial vector for GST fusion?

Bacterial vector for expressing GST fusion proteins with a PreScission protease site. For other reading frames, use pGEX-6P-2 or pGEX-6P-3. Bacterial vector for expressing GST fusion proteins with a PreScission protease site.

Which is the best reading frame for pgex-4t-2?

For other reading frames, use pGEX-4T-2 or pGEX-4T-3. Bacterial vector for expressing fusion proteins with a thrombin site. For other reading frames, use pGEX-4T-2 or pGEX-4T-3. FEATURES VIEWER PLASMIDS RESOURCES PRICING MY ACCOUNT FREE TRIAL FEATURES VIEWER PLASMIDS RESOURCES PRICING MY ACCOUNT FREE TRIAL Plasmid Files

Which is the correct sequence for expressing GST fusion proteins?

Bacterial vector for expressing GST fusion proteins with a PreScission protease site. For other reading frames, use pGEX‑6P-2 or pGEX‑6P-3. To see this sequence with restriction sites, features, and translations, please download SnapGene or the free SnapGene Viewer.