What does FOA do?

The FOA is an international non-profit educational organization that is chartered to promote professionalism in fiber optics through education, certification and standards.

Can you autoclave 5-FOA?

Combine the ingredients above, and add distilled water to give a total volume of 500 mL. Filter-sterilize using a 0.2-µm filter. Dissolve 10 g of bacto-agar in 500 mL of distilled water, autoclave, and cool to ∼80°C.

What does FOA stand for Military?

Field operating agencies (FOAs) perform field activities beyond the scope of any of the major commands. Their activities are specialized or associated with an Air Force wide mission.

What does Foa mean in law?

Fax of Acceptance
FOA means Fax of Acceptance for notification of Award.

What is the purpose of 5-fluorouracil 6-carboxylic acid monohydrate?

Thermo Scientific 5-Fluoroorotic Acid (5-fluorouracil-6-carboxylic acid monohydrate; 5-FOA) is used in yeast molecular genetics to detect expression of the URA3 gene, which encodes orotine-5′-monophosphate (OMP) dicarboxylase. Yeasts with an active URA3 gene (Ura+) convert 5-FOA to fluorodeoxyuridine, which is toxic to cells (see the scheme below).

How is 5-fluoroorotic acid toxic to yeast?

Otherwise nontoxic to yeast, 5-FOA is converted to the toxic form (i.e., 5-flurouracil) in strains expressing the functional URA3 gene coding for orotine-5-monophosphate decarboxylase that is involved in the synthesis of uracil. Yeast strains that are phenotypically Ura+ become Ura- and 5-FOA (R) (resistant) after selection.

How is the purity of 5-fluorotic acid determined?

Convenient: Available as a pure powder or ready-to-use solution in DMSO. Ultra-Pure: Determined > 98% by thin-layer chromatography (TLC), melting point, and lot comparison. Using 5-Fluoroorotic Acid (5-FOA) for the counter-selection of yeast is a common genetic screening method.

What kind of yeast converts 5 FOA to fluorodeoxyuridine?

Yeasts with an active URA3 gene (Ura+) convert 5-FOA to fluorodeoxyuridine, which is toxic to cells (see the scheme below).