How is RNase A stock solution prepared?

To prepare a 10 mg/mL RNase A stock solution, dissolve 100 mg of RNase A in 10 mL of Tris-Cl (10 mM, pH 7.5)/NaCl (15 mM). Heat to 100ºC for 5 min and cool at room temperature. Store at −20ºC.

What is the function of RNase A in a DNA extraction?

RNase A is an endoribonuclease that specifically hydrolyzes RNA 3´ of pyrimidine residues and cleaves the phosphodiester linkage to the adjacent nucleotide. RNase A is used to remove RNA during procedures for the isolation of plasmid and genomic DNA.

How do you use RNase solution?

You simply spray RNaseZap® Solution onto the surface to be decontaminated and rinse it off with RNase-free water. Working with RNA requires that special measures be taken to ensure an RNase-free environment.

How to make a stock solution from RNase?

The solution can be prepared by simply dissolving RNase in deionized or distilled water; however, the RNase will occasionally precipitate from the solution and activity will be lost. 4 M Sodium Chloride (NaCl) Makes 500 ml. Store at room temperature (indefinitely). 1. Dissolve 116.9 g of NaCl (m.w. 58.44) in 250 ml of deionized or distilled water.

How does Sigma test the activity of RNase A?

When Sigma tests the activity of RNAse A, a stock solution is prepared in water at 1 mg/ml. Solutions prepared from powdered RNase A products can be made free of DNase by boiling. According to a literature method,8prepare a 10 mg/mL stock solution in 10 mM sodium acetate buffer, pH 5.2.

What kind of reagent to use for RNase A?

4OAc) Edward’s Buffer 0.5 M Ethylene Diamine Tetraacetic Acid (EDTA) Phenol:chloroform (2:1; volume:volume) 5 mg/ml RNase A (Pancreatic RNase) 4 M Sodium Chloride (NaCl) 10% Sodium Dodecyl Sulfate (SDS) 1 M Tris (pH 8.0) Tris/EDTA (TE) Buffer Tris/EDTA Buffer with RNase A (TER) Urea Extraction Buffer. II.

How to make RNase A from glacial acetic acid?

1. Dissolve 100 mg of RNase A in 20 ml of 0.05% glacial acetic acid, and transfer to a 50-ml conical tube. 2. Place the tube in a boiling-water bath for 15 minutes. 3. Cool the solution, and neutralize by adding 120 ml of 1 M Tris (pH 8.0).