What is a glucose oxidase assay?

The glucose oxidase enzyme (GOx or GOD) also known as notatin (EC number 1.1. 3.4) is an oxidoreductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone.

How does glucose oxidase work?

Glucose oxidase utilizes oxygen by catalysing the oxidation of β-D-glucose to produce 2-δ-gluconolactone and hydrogen peroxide. The gluconolactone is spontaneously hydrolysed to D-gluconic acid, and the hydrogen peroxide can be removed by catalase.

What is the role of glucose oxidase?

Glucose oxidase enzymes are used to remove small amounts of oxygen from food products or glucose from diabetic drinks. Glucose oxidase is playing an important role in color development, flavor, texture, and increasing the shelf life of food products (Khurshid et al., 2011).

Which is the best glucose oxidase assay kit?

The Glucose Oxidase assay kit is a simple procedure for the rapid and reliable measurement and analysis of glucose oxidase activity in industrial enzyme preparations and bread improver mixtures.

How is glucose oxidized in a colorimetric assay?

In this assay, glucose is oxidized to δ-gluconolactone with concomitant reduction of the flavin adenine dinucleotide (FAD)-dependent enzyme glucose oxidase (see Figure 1 on page 7; reaction 1). The reduced form of glucose oxidase is regenerated to its oxidized form by molecular oxygen to produce hydrogen peroxide (reaction 2).

How is glucose oxidase used to calculate blood glucose?

Glucose oxidase is an enzyme extracted from the growth medium of Aspergillus niger. Glucose oxidase catalyse the oxidation of Beta D- glucose present in the plasma to D glucono -1 ,5 – lactone with the formation of hydrogen peroxide; the lactone is then slowly hydrolysed to D-gluconic acid.

Which is the best method for enzymatic glucose analysis?

The enzymatic glucose analyses are based on the two methods most commonly employed in clinical chemistry, the “hexokinase method” [ 5, 6] and the “glucose oxidase method” [ 7 ]. Both methods employ a single premixed solution to perform the reaction, which may be purchased in kit form.