How do you calculate pipette calibration?
How do you calculate pipette calibration?
Calculate the accuracy of the pipette.
- If the pipette is properly calibrated, the calculated value should be very close to the actual value you set on the pipette.
- For example: A = 100 x Vavg/V0 = 100 x 10.019/10 = 100 x 1.0019 = 100.19%
- This particular pipette is properly calibrated.
What is the Z factor in pipette calibration?
The calibration of pipette is carried out by gravimetric method. When determining the volume of water, the accuracy of measurements is effected by ambient temperature, atmospheric pressure and relative humidity. These factors are usually combined to give the Z factor, used in calculation of volume of water.
How do you calculate calibration volume?
Calculate the dispensed volume by using the equation V = W x Z where W is the weight of the water, Z is the Z factor, and V is the calculated volume of dispensed water. Next, determine the mean value from ten trials.
How much does it cost to calibrate a pipette?
Pipette Calibration Systems Cost Analysis
| Pipette Calibration System | Initial one time cost |
|---|---|
| Gravimetric | Analytical balance (Estimate $3000-$4000) |
| Photometric | Spectrophotometer (Estimate $1500 -$2,000) |
| Artel- PCS | PCS instrument ($6,500 – 7,000) |
| Streck Pipette Verification Service | None |
How much inaccuracy is required for a Pipet calibration?
% Inaccuracy must be less than 2.00 *Created by the Wisconsin State Laboratory of Hygiene Annual Pipette Calibration (gravimetric)* Quarterly Pipette Calibration (gravimetric)* xxx assd 100.00 100.00 100.00
How to calculate the mean weight of a pipette?
Calculations used for Verifying Pipette Calibration Mean Weight = sample replicate 1 + sample replicate 2 + (etc.) Number of Replicates Mean Volume (Corrected Mean) = Mean Weight × ZFactor % Inaccuracy = [(Corrected Mean – True Value) ÷ True Value] × 100
How do you do Pipette calibration on a scale?
Dispense the liquid into the weigh boat on the scale. Place the tip against the bottom of the weigh boat and push the button down to the first stop. Move to another spot slightly away from the water and push the button to the second stop. Keeping the button pressed, lift the tip away from the weigh boat.
Can You calibrate a micropipette by yourself?
Calibration of the balance may be done by an external service agent or by yourself. The balance uncertainty contributes quite (if not most) significantly to the calibration of a pipette at lower volumes (typically below 100 µl). If you calibrate the balance yourself, use a set of mass pieces ranging from 1 mg to 100 g.
https://www.youtube.com/watch?v=yNCAJhXhf8I