What is a T4 DNA ligase?

Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′-phosphate and 3′-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. T4 DNA Ligase requires ATP as a cofactor.

What is function of T4 DNA ligase?

T4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. The enzyme will not join single-stranded nucleic acids.

Where does T4 DNA ligase come from?

T4. The DNA ligase from bacteriophage T4 (a bacteriophage that infects Escherichia coli bacteria). The T4 ligase is the most-commonly used in laboratory research. It can ligate either cohesive or blunt ends of DNA, oligonucleotides, as well as RNA and RNA-DNA hybrids, but not single-stranded nucleic acids.

How big is Promega T4 DNA ligase buffer?

Promega products. T4 DNA Ligase: Size Part No. (Weiss units) M180A 100 M180B 500 M179A (High Conc.) 500 Ligase Buffer, 10X (C126A, C126B): The Ligase 10X Buffer supplied with this enzyme has a composition of 300mM Tris-HCl (pH 7.8), 100mM MgCl 2, 100mM DTT and 10mM ATP. The performance of this buffer depends on the integrity of the ATP.

Where can I get a T4 DNA ligase?

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab’s needs. T4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides.

How does T4 DNA ligase repair single stranded nicks?

This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids (1). T4 DNA ligase will seal nicks for these DNA substrates. Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart Quick Tips – Do I need a 5′ phosphate for ligation?

What is the role of T4 in DNA ligation?

T4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides.