What is simulated gastric fluid?

Artificial gastric fluid (Simulated Gastric Fluid) is a solution that simulates the composition and pH of gastric juice. The reagent is sterilized by 0.2 μm filtration. Artificial gastric juice is mainly composed of sodium chloride, dilute acid, pepsin, etc.

How do you get simulated intestinal fluid?

The simulated intestinal fluid was prepared by the similar method: potassium phosphate monobasic (10.2 g) and SDS (3.75 g) were dissolved in a same 1000 ml of deionized water and then pH adjusted to 6.8 ± 0.1 with 1 N NaOH. Finally, the volume of each fluid was adjusted to 1500 (ml) with deionized water.

What is FaSSIF and FeSSIF?

FaSSIF: Fasted State Simulated Intestinal Fluid. FeSSIF: Fed State Simulated Intestinal Fluid.

What is FaSSGF?

FaSSGF: Fasted State Simulated Gastric Fluid. These media are incredibly useful tools when developing an oral drug because simple laboratory experiments in them can help predict how the drug is likely to perform in the gut.

How is fasted state simulated gastric fluid created?

A medium representing the fasted conditions in a human stomach, so-called Fasted-State Simulated Gastric Fluid (FaSSGF), was proposed by Vertzoni et al. in 2005 (5). The medium was designed to embrace the important aspects of human basal gastric juice plus a glass of water normally given with a dosage form.

What are the pH values of fedgas gastric fluid?

FEDGAS is available at 3 different pH values: 6, 4.5 and 3 (each sold separately). Stir until mixed thoroughly. The medium will be white like milk and is ready to use Stir until mixed thoroughly. The medium will be white like milk and is ready to use Stir until mixed thoroughly. The medium will be white like milk and is ready to use

What kind of media is used to simulate gastric conditions?

Simulated Gastric Fluid The traditional medium to simulate gastric conditions in the fasted state has been simulated gastric fluid (SGF) of the USP. This medium contains hydrochloric acid and sodium chloride, as well as pepsin and water, and has a pH of 1.2.

What’s the purpose of simulating small intestinal fluid?

The purpose of this work is to evaluate the roles of lecithin and bile salts in a new generation of fasted simulated small intestinal fluid (FaSSIF-II), thus enhancing the closer mimic of simulated fluids to the real human intestinal fluids (HIF) in drug discovery and drug product development.