What is the wavelength of luminescence?

The excitation spectra are different from the absorption spectra and all the samples have absorption extended to 200 nm or even shorter. So, the excitation at 200–300 nm range can produce luminescence.

Does phenol red interfere with luminescence?

The luminescence signal also can be affected by changes in temperature or the presence of phenol red and organic solvents (Section 5.B). ! Because the luminescent signal is affected by assay conditions, results should be compared only between samples measured using the same medium/serum combination.

How does CellTiter Glo work?

The CellTiter-Glo™ Luminescent Cell Viability Assay(a) uses ATP, a required co-factor of the luciferase reaction, as an indicator of metabolically active cells (1–3). The enzyme luciferase acts on luciferin in the presence of Mg2+ and ATP to produce oxyluciferin and to release energy in the form of luminescence (4).

What does cell titer glo measure?

ATP
CellTiter-Glo® Measures ATP, A Key Biomarker of Cell Health. The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells.

What kind of assay is celltiter Glo used for?

The CellTiter-Glo® Assay is designed for use with multiwell plate formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays.

How is the viability of a luminescent cell determined?

The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method to determine the number of viable cells in culture based on quantitation of the ATP present, which signals the presence of metabolically active cells.

How many PC-3 cells are used in cell titration?

For cell titration experiments, a range of PC-3 cell concentrations were used up to 36,000 cells/well. After 24 hours, reagent was added according to manufacturer’s specifications and read on the Synergy LX using the detection modes described above.

How is luminescence determined on a microplate?

The plate was shaken on an orbital shaker for 2 minutes then incubated for 10 minutes in the dark at room temperature within the Synergy LX reader prior to luminescence determination using a Synergy LX with a dedicated luminescence cube. This cube allows direct passage of light from the microplate well to the PMT detector.