What is the purpose of the Kirby-Bauer disk diffusion test?

The purpose of the Kirby-Bauer disk diffusion susceptibility test is to determine the sensitivity or resistance of pathogenic aerobic and facultative anaerobic bacteria to various antimicrobial compounds in order to assist a physician in selecting treatment options for his or her patients.

How do you calculate the zone of inhibition in disc diffusion?

To measure the zone of inhibition, first place the plate on a non-reflective surface. Take a ruler or caliper that measures in millimeters and place the “0” in the center of the antibiotic disk. Measure from the center of the disk to the edge of area with zero growth. Take your measurement in millimeters.

What is Kirby-Bauer disk diffusion?

The Kirby-Bauer disk diffusion susceptibility test determines the sensitivity or resistance of pathogenic bacteria to various antimicrobial compounds in order to assist physicians in selecting treatment options their patients.

What is the zone of diffusion?

The diffusion zone morphology, which develops during solid-state interaction in a ternary couple, is defined by type, structure, number, shape and topological arrangement of the newly formed phases. The resulting microstructure of the reaction zone can be visualized with the aid of the so-called diffusion path.

What is Kirby Bauer procedure?

Procedure for Modified Kirby Bauer method Prepare the inoculum from the primary culture plate by touching with a loop the tops of each of 3 – 5 colonies, of similar appearance, of the organism to be tested and transfer this growth to a tube of saline.

What is Kirby Bauer antibiotic test?

Kirby-Bauer antibiotic testing (also called KB testing or disk diffusion antibiotic sensitivity testing) uses antibiotic-containing wafers or disks to test whether particular bacteria are susceptible to specific antibiotics. First, a pure culture of bacteria is isolated from the patient.

What is Kirby Bauer technique?

The Kirby-Bauer technique is a carefully standardized single-disk-diffusion test of susceptibility to antibiotics. Interpretative categories have been developed from a synthesis of zone size with MIC-blood level, or in some cases, correlations with urinary levels, susceptibility distribution of bacterial populations, and clinical experience.

What is disk diffusion method?

Disk-Diffusion Method. The disk-diffusion method involves applying different chemicals to separate, sterile filter paper disks. The disks are then placed on an agar plate that has been inoculated with the targeted bacterium and the chemicals diffuse out of the disks into the agar where the bacteria have been inoculated.