What is RNA interference PPT?

Definition RNA interference (RNAi) is a mechanism that inhibits gene expression at the stage of translation or by hindering the transcription of specific genes. …

What is RNA interference therapy?

RNA interference (RNAi) is a mechanism for gene silencing. Such mechanism possesses uncanny ability in targeting cancer-related genes. A majority of gene products involved in tumorigenesis have recently been utilized as targets in RNAi based therapy.

What are the steps in RNA interference?

In the first step, the trigger RNA (either dsRNA or miRNA primary transcript) is processed into an short, interfering RNA (siRNA) by the RNase II enzymes Dicer and Drosha. In the second step, siRNAs are loaded into the effector complex RNA-induced silencing complex (RISC).

What is the mechanism of RNA interference ( RNAi )?

Mechanism of RNA interference (RNAi)  dsRNA are chopped into short interfering RNAs (siRNA) by Dicer.  2. The siRNA-Dicer complex recruits additional components to form an RNA-Induced Silencing Complex (RISC). The siRNA unwinds.  3. The unwound siRNA base pairs with complementary mRNA, thus guiding the RNAi machinery to the target mRNA.  4.

How is RNA interference used to silence genes?

RNAi is a powerful, conserved biological process through which the small, double-stranded RNAs specifically silence the expression of homologous genes, largely through degradation of their cognate mRNA. 1. RNA interference RNAi BY Kamlesh Kumar Chandel Ph.D. Scholar Department of Genetics and Plant Breeding 2. Content I. Overview view / History II.

What are the therapeutic uses of RNA interfernce?

Therapeutic uses of RNAi Hematology (blood) Oncology (cancer) Stem cell biology Infectious diseases 29. Hematology (blood) Hematologic disorders result from Loss of gene function Mutant gene function Absent gene function RNAi May be used to create models of disease processes Could help to develop pharmacologic and genetic therapeutic targets

Who is the Nobel Prize winner for RNA interference?

1. RNA interference RNAi BY Kamlesh Kumar Chandel Ph.D. Scholar Department of Genetics and Plant Breeding 2. Content I. Overview view / History II. Mechanism / Process III. Function IV. Applications V. RNAi Glossary VI. References Nobel Prize in 2006 3.