When to add DNA to FuGene 6 transfection?

Add an appropriate amount of DNA to FuGENE®6 Transfection Reagent/ medium to achieve the proper ratio of reagent to DNA. Mix and incubate for 15 minutes. Plate cells one day before the transfection experiment so that cells will be approximately 50–80% confluent on the day of transfection.

Which is better FuGene 6 or other reagents?

Compared to other transfection methods, FuGENE 6 Transfection Reagent gives more consistent results, saves time and effort, and has minimal effect on cell physiology. Delivery of nucleic acids into cells using liposomal or multicomponent transfection reagents is a well-established method for transfection of mammalian cell lines.

How does the FuGene HD transfection reagent work?

The FuGENE® HD Transfection Reagent is a nonliposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex.

Can you store FuGene 6 in a glass vial?

Do not freeze or store below 0°C. Formulation and Packaging: FuGENE® 6 Transfection Reagent is a proprietary mixture of lipids and other components in 80% ethanol, fi ltered through a 0.1µm fi lter and supplied in glass vials.

Which is better for reverse transfection Lipofectamine 2000 or FuGene 6?

We found that FuGENE® 6 and FuGENE® HD performed as well as or better than competitor transfection reagents in a “reverse transfection” experiment and were less toxic to cells than Lipofectamine™ 2000. The ability to transfect a cell with DNA is key to many biological assays that study such things as protein function and intracellular events.

What is the reagent DNA ratio for FuGene 6?

This is equivalent to over 3,300 wells in 96-well plates at a 3:1 FuGENE®6 Transfection Reagent:DNA ratio (0.3µl reagent:100ng DNA per well). The actual number of transfections will vary with reagent:DNA ratio, transfection volume and cell type. Storage Conditions: Store FuGENE®6 Transfection Reagent at 4°C. Close lid tightly after use.

Which is the best reagent for reverse transfection?

There are a wide variety of transfection reagents available to help the researcher reproducibly transfect the DNA. The FuGENE® 6 and FuGENE® HD Transfection Reagents are nonliposomal reagents that transfect DNA into a wide variety of cell types with high efficiency and low toxicity.