Which protein assay is the best?

Top 5 Protein Quantification Assays

• Bicinchoninic Acid (BCA) This colorimetric, two-step assay was originally developed in 1985 – making it a baby compared with the 64-year-old Lowry assay!
• Bradford.
• Folin-Lowry.
• Kjeldahl.
• Ultraviolet Absorption.

What is protein assay used for?

Purpose of Protein Assays The purpose of the protein assay is to determine the amount or concentration of a specific protein or an array of different proteins a sample.

Which technique is used for protein assay?

The simplest and most direct assay method for protein concentration determination in solution is to measure the absorbance at 280 nm (UV range). Amino acids containing aromatic side chains (i.e., tyrosine, tryptophan and phenylalanine) exhibit strong UV-light absorption.

How much protein is in a Bio Rad assay?

Choose the Bio-Rad Protein Assay for simple, accurate measurement of total protein. This colorimetric assay based on the Bradford method is compatible with many common lab reagents. Use the standard assay for protein concentrations between 200 and 1,400 µg/ml (20–140 µg total protein)

Which is colorimetric assay kit does Bio-Rad use?

Colorimetric assay kit, includes 450 ml dye reagent concentrate, bovine serum albumin standard; for 440 standard assays or 2,200 microplate assays What is the education discount policy? For more than 20 years, Bio-Rad has made science education a major priority.

Where can I get a protein assay kit?

The Bradford protein assay kit was from Bio-Rad (Hercules, CA); nitrocellulose membranes were from Schleicher and Schuell (Keene, NH). .. The rabbit anti-Synechococcus PCC6301 antibodies were the generous gift of Robert Tabita (Ohio State University).

What should be the reagent concentration for Bio-Rad?

The reagent concentrations given in the reagent compatibility table are for the standard assay procedure (0.2–1.4 mg/ml protein). Concentrations compatible with the microassay procedure (1.25–25 µg/ml protein) are 1/40 of those listed.