What is RNase Zap?

RNaseZap® completely removes RNase contamination from glass and plastic surfaces. It contains three different ingredients known to be active against RNase. It effectively removes high levels of RNase contamination that similar products cannot. RNaseZap has been used to remove RNase contamination from reaction vessels.

How does RNA zap work?

RNaseZap® RNase Decontamination Solution is a surface decontamination solution that destroys RNases on contact. You simply spray RNaseZap® Solution onto the surface to be decontaminated and rinse it off with RNase-free water. Working with RNA requires that special measures be taken to ensure an RNase-free environment.

How do I use RNaseZap?

Apply RNaseZap Solution liberally to a paper towel and wipe all exposed surfaces of the apparatus thoroughly. Rinse with water and then wipe dry. Some small parts may be cleaned by briefly soaking them in RNaseZap Solution, rinsing them with water and then drying.

What causes RNA contamination?

The major sources of RNase contamination in a typical laboratory include: aqueous solutions, such as the reagents used in experiments; environmental exposure caused by, for example, airborne RNases, untreated surfaces and dust particles; and human contact with hands and skin.

Does RNase zap remove DNA?

RNaseZap® RNase Decontamination Solution is a surface decontamination solution that destroys RNases on contact. You simply spray RNaseZap® Solution onto the surface to be decontaminated and rinse it off with RNase-free water.

How do you test for RNase contamination?

The most common method for detecting RNase contamination in solutions is to incubate an RNA substrate with a test solution and then check for degradation of the RNA by ethidium bromide stained agarose gel electrophoresis. This assay has very low sensitivity and requires a subjective judgment.

How is DNA RNA contamination detected?

The Acclaro software that runs the Nanodrop One spectrophotometer allows the identification of RNA contamination in a DNA sample and provides a corrected concentration result. These two factors will allow molecular biologists to quickly troubleshoot difficult extractions and improve downstream results.

How much RNase A to add?

Recommended concentration of RNase A is 1 to 100 µg/mL depending on the application. The enzyme is active under a wide range of reaction conditions. At low salt concentrations (0 to 100 mM NaCl), RNase A cleaves single-stranded and double-stranded RNA as well the RNA strand in RNA-DNA hybrids.

Can RNase degrade DNA?

RNase A does not degrade DNA but can bind to DNA [25]. If the formation of RNase A-DNA complexes is required for the observed DNA removal, then DNA removal should be inhibited by the presence of excess DNA.

How is RNase Zap used for surface decontamination?

See alternate available products . RNaseZap™ RNase Decontamination Solution is a surface decontamination solution that destroys RNases on contact. You simply spray RNaseZap™ Solution onto the surface to be decontaminated and rinse it off with RNase-free water.

Is it safe to wipe with RNase Zap?

It is highly recommended as it completely wipes out the RNAse and the experiment can be run safely. No need to risk time, money and samples. So, to sum up, it is not a detergent, but a solution that denatures proteins.

What makes RNase Zap turn into H 2 O?

A lot of “home made RNaseZap” recipes on the internet include hydrogen peroxide, which causes a variety of covalent modifications to the protein. It also has the advantage of cleaning itself up (eventually turning into H 2 O).

What to use to remove RNase from surfaces?

All surfaces, including benchtops, pipettes, glassware, and benchtop instruments, should be treated with a surface decontamination agent. We offer several surface decontamination products that are proven effective at eliminating RNase contamination from lab surfaces— RNaseZap Solution, RNaseZap Wipes, ElectroZap Solution, and RNase AWAY Reagent.