What is the purpose of the enzyme experiment?

You will design a set of experiments to examine the effects of temperature, pH, and substrate concentration on the ability of enzymes to catalyze chemical reactions.

What affects enzyme activity lab?

An enzyme can be denatured (unfolded) by extreme heat, pH, or ionic concentration. If this happens, the enzyme will no longer be functional because the shape of the active site will be destroyed.

What is the enzyme in the enzyme lab?

In this lab, you will study an enzyme that is found in the cells of many living tissues. The name of the enzyme is catalase; it speeds up a reaction which breaks down hydrogen peroxide, a toxic chemical, into 2 harmless substances–water and oxygen.

What are the factors that affect enzyme activities?

Factors affecting enzyme activity Enzyme activity can be affected by a variety of factors, such as temperature, pH, and concentration. Enzymes work best within specific temperature and pH ranges, and sub-optimal conditions can cause an enzyme to lose its ability to bind to a substrate.

How does pH affect enzyme activity lab?

With a higher Ea, the reaction rate is very slow. This is what altering the pH of the solutions did. It altered the tertiary structure of the enzyme, slowing the reaction rate. Altering the pH of the solution altered the tertiary structure of the enzyme by changing how the side chains bonded with each other.

What are the two methods for measuring enzymatic activity?

The methods used for measuring enzymatic activities include spectrophotometry, fluorescence, and radiolabeling. The enzymatic assay can be direct or indirect; where, in the case of direct assay substrate is added to the soil system and the end product formed is determined.

What are 4 factors that can regulate enzyme activity?

There are many factors that can regulate enzyme activity, including temperature, activators, pH levels, and inhibitors. Temperature: That’s a good one. Proteins change shape as temperatures change.