What is anti Digoxigenin?

Sheep anti digoxigenin antibody recognizes digoxigenin, a steroid found in plants that is often attached to oligonucleotides and used as a molecular probe to detect mRNA production. This antibody cross reacts with digoxin and digitoxin. Negligible reactivity has been observed with ouabain.

What is digoxigenin used for?

Digoxigenin is widely used as a method for generating non-radioactive probes, and is an alternative to biotinylation. Like biotin, DIG-labeled probes support both chromogenic and chemiluminescence detection formats.

Is digoxigenin a protein?

DIG-binding proteins. Tinberg et al. 3, was a 141 amino acid protein that bound DIG with a dissociation constant (Kd) of 541 (+/- 193) pM. Anti-digoxigenin antibodies with high affinities and specificity are used in a variety of biological immuno-assays (e.g. ELISA).

What is dig labeling?

The DIG System is the nonradioactive technology of choice to label and detect nucleic acids for multiple applications. The system is based on a steroid isolated from digitalis plants (Digitalis purpurea and Digitalis lanata).

Is situ a hybridization?

In situ hybridization (ISH) is a powerful technique for localizing specific nucleic acid targets within fixed tissues and cells, allowing you to obtain temporal and spatial information about gene expression and genetic loci.

What is non radioactive probe?

The most commonly used labels for the generation of non-radioactively DNA or RNA hybridization probes are fluorophores and haptens, the latter meaning Biotin and Digoxigenin. Fluorescent probes are detected directly after incorporation by fluorescence spectroscopy (Fig. 2A).

What is Northern blotting technique?

Northern Blotting is a technique used for the study of gene expression. It is done by detection of particular RNA (or isolated mRNA). This method reveals the identity, number, activity, and size of the particular gene. This blotting technique can also be used for the growth of a tissue or organism.

What is biotin labeling?

Biotinylation, also known as biotin tagging/labeling, is the process of attaching biotin to a protein molecule and other macromolecules.

What is a dig?

The definition of a dig is a sarcastic remark or joke, or an archaeological search. An example of a dig is a teasing comment made at someone. An example of a dig is a search for dinosaur bones in the ground. noun.

Why situ hybridization is used?

In situ hybridization is used to reveal the location of specific nucleic acid sequences on chromosomes or in tissues, a crucial step for understanding the organization, regulation, and function of genes. Fluorescent DNA ISH (FISH) can, for example, be used in medical diagnostics to assess chromosomal integrity.

How is in situ hybridization used?

In situ hybridization is a technique that is used for localization and detection of specific DNA and RNA sequences in cells, preserved tissue sections, or entire tissue (whole mount in situ hybridization, Fig. 1) by hybridizing the complementary strand of a nucleotide probe to a particular sequence.

What is hybridization techniques?

Hybridization is the process of combining two complementary single-stranded DNA or RNA molecules and allowing them to form a single double-stranded molecule through base pairing. Hybridization is a part of many important laboratory techniques such as polymerase chain reaction and Southern blotting.

Which is the best Antibody to treat digoxigenin?

Digoxigenin Antibodies. Digoxigenin (DIG), a steroid, is a derivative of the flowers and leaves of plants, i.e., Digitalis sp. Due to the high immunigenicity of this small molecule it is highly preferred in many molecular biology applications.

Where is digoxigenin found in the human body?

Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases.

How is horseradish peroxidase used for digoxigenin detection?

Recognition of digoxigenin (DIG) provided by anti-digoxigenin antibody makes it very useful for detection in a variety of assays. The horseradish peroxidase (HRP) conjugate of the antibody is ideal for use with high sensitivity detection substrates like TSA and Western Lightning.

What kind of heart disease is digoxin used for?

Digoxin is widely used in the treatment of various heart conditions, namely atrial fibrillation, atrial flutter and sometimes heart failure that cannot be controlled by other medication. Digoxin preparations are commonly marketed under the trade names Lanoxin, Digitek, and Lanoxicaps.