What is MLST used for?

Multilocus sequence typing (MLST) was first proposed in 1998 as a typing approach that enables the unambiguous characterization of bacterial isolates in a standardized, reproducible, and portable manner using the human pathogen Neisseria meningitidis as the exemplar organism.

How does MLST work?

MLST directly measures the DNA sequence variations in a set of housekeeping genes and characterizes strains by their unique allelic profiles. In the data analysis step, all unique sequences are assigned allele numbers and combined into an allelic profile and assigned a sequence type (ST).

What is MLST in bacteria?

Multilocus sequence typing (MLST) is an unambiguous procedure for characterising isolates of bacterial species using the sequences of internal fragments of (usually) seven house-keeping genes.

What is MLST analysis?

Multilocus sequence typing (MLST) is a molecular typing technique whereby a number of well chosen housekeeping genes (loci) are sequenced, usually in part. In a typical MLST approach, recombination is expected to occur with a much higher frequency than point mutations.

What is BIGSdb?

BIGSdb is software designed to store and analyse sequence data for bacterial isolates. BIGSdb extends the principle of MLST to genomic data, where large numbers of loci can be defined, with alleles assigned by reference to sequence definition databases (which can also be set up with BIGSdb).

What is the importance of multilocus sequence typing?

Multilocus sequence typing (MLST) has become a useful tool for studying the genetic diversity of important public health pathogens, such as Chlamydia trachomatis (Ct). Four MLST schemes have been proposed for Ct (data available from Chlamydiales MLST databases).

What is core genome MLST?

The aim of Core Genome MLST (cgMLST) is to extend the MLST concept to a larger number of genes of the core genome. It relies on WGS but allows for the examination of a fixed number of genome loci and the creation of a systematic allele numbering system.

What is EnteroBase?

EnteroBase 1 is a Powerful, User-Friendly Online Resource for Analyzing and Visualizing Genomic Variation within Enteric Bacteria.

What is DNA genotyping?

Genotyping determines differences in genetic complement by comparing a DNA sequence to that of another sample or a reference sequence. It identifies small variations in genetic sequence within populations, such as single-nucleotide polymorphisms (SNPs).

Why do we need housekeeping genes?

Housekeeping genes are typically constitutive genes that are required for the maintenance of basal cellular functions that are essential for the existence of a cell, regardless of its specific role in the tissue or organism.

How are PCR machines used in medical testing?

Several testing tools have been used to determine the number of infected persons. The PCR (Polymerase Chain Reaction) machine has been of great significance in his exercise. It uses the principle of genetic tracing and amplification of DNA and RNA strands located in liquid surfaces.

How are nucleotide differences between strains checked in MLST?

Nucleotide differences between strains can be checked at a variable number of genes depending on the degree of discrimination desired. The workflow of MLST involves: 1) data collection, 2) data analysis and 3) multilocus sequence analysis.

Is the MultiLocus Sequence Typing ( MLST ) scalable?

Among these techniques emerges the Multilocus Sequence Typing (MLST) ( Klint et al., 2007; Pedersen et al., 2008; Bom et al., 2011; Xia and Xiong, 2014; de Vries et al., 2015) that has provided a portable, reproducible and scalable typing system and is performed easily by different laboratories ( Urwin and Maiden, 2003 ).

Are there any standardized MLST schemes for CT?

Four MLST schemes have been proposed for Ct (data available from Chlamydiales MLST databases). However, the lack of a sole standardized scheme represents the greatest limitation regarding typing this species.