What type of detectors are used in HPLC?

HPLC Detectors

• UV-Vis Detectors. The SPD-20A and SPD-20AV are general-purpose UV-Vis detectors offering an exceptional level of sensitivity and stability.
• Refractive Index Detector.
• Fluorescence Detectors.
• Evaporative Light Scattering Detector.
• Conductivity Detector.

What is difference between PDA and UV?

There are detectors that provide wider wavelength selection, covering both UV and VIS ranges (195~700 nm) called UV/VIS detector. PDA detects an entire spectrum simultaneously. UV and VIS detectors visualize the obtained result in two dimensions (light intensity and time), but PDA adds the third dimension (wavelength).

What is RI detector in HPLC?

The 2414 Refractive Index (RI) Detector is designed for high-performance liquid chromatography (HPLC) applications. It provides sensitivity, stability, and reproducibility for the analysis of components with limited or no UV absorption.

Why is UV light used in HPLC?

HPLC UV detectors are used with high performance liquid chromatography to detect and identify analytes in the sample. A UV visible HPLC detector uses light to analyze samples. By measuring the sample’s absorption of light at different wavelengths, the analyte can be identified.

What is C4 column?

YMC-Pack C4 (Butyl) columns are less hydrophobic than C8 or C18 packings, and generally utilize more aqueous eluents than either of these reversed phase column types. When compared to C8 and C18 columns using the same eluent, C4 columns show significantly shorter retention for non-polar compounds.

What are diode array detectors?

Diode-Array Detection (DAD) or Photodiode-Array Detection (PDA) is an analytical technique that can be used to determine the purity of an analyte or related impurity peak eluting during an HPLC separation. The use of DAD to demonstrate method specificity is allowed by the International Council for Harmonisation (ICH) guideline Q2 (R1).

What is HPLC PDA detector?

HPLC PDA Detector Diode array detectors (also referred to as a DAD detector or more specifically HPLC PDA detector) are used for obtaining spectral profiles from molecular mixtures or chromatographically separated samples.

What is a photodiode array (PDA)?

A photodiode array (PDA) is a linear array of discrete photodiodes on an integrated circuit (IC) chip . For spectroscopy it is placed at the image plane of a spectrometer to allow a range of wavelengths to be detected simultaneously. In this regard it can be thought of as an electronic version of photographic film.