How many kDa is beta-actin?

42 kDa
Beta-Actin (42 kDa) is commonly chosen as a loading control due to its general expression across all eukaryotic cell types.

What is the size of beta-actin?

beta-actin – a housekeeping protein Though beta-actin has a molecular weight of 42 kDa, degradation will produce 35–38 kDa, ~ 30 kDa, 15 kDa, and other typical fragments on a Western blot, of which 35-38 kDa fragments are the most common.

What is the size of actin protein?

approximately 7 nm
The major cytoskeletal protein of most cells is actin, which polymerizes to form actin filaments—thin, flexible fibers approximately 7 nm in diameter and up to several micrometers in length (Figure 11.1).

What does β-actin do?

Beta-actin, also known as a “housekeeping” protein, is usually used as a loading control , for among others, the integrity of cells, protein degradation, in PCR and Western blotting. They can be used to normalize the levels of protein detected by confirming that protein loading is the same across the gel.

What kind of protein is actin alpha 1?

Actin, alpha skeletal muscle is a protein that in humans is encoded by the ACTA1 gene. Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity.

What are beta actin and gamma actin proteins?

Beta and gamma 1 are two non-muscle actin proteins. They function as the main components of microfilaments (Figure 2). Actins are highly-conserved. Beta-actin proteins from species as diverse as human, mouse, and chicken are identical. Human beta actin shares close to 90% identity with fungal homologs.

Is there an antibody that recognises beta actin?

Research with confidence – consistent and reproducible results with every batch This antibody recognises beta and gamma actin in Human samples. It probably also recognises all the other known forms of Human actin. This antibody detects a single clean band in Human, Mouse, Rat, Chicken and Drosophila samples.

What are the three main groups of actins?

In humans, three main actin groups have been identified – alpha, beta, and gamma. The alpha actins are expressed primarily in skeletal, cardiac, and smooth muscle, while the remaining beta and gamma.