What does Flipr stand for?

Fluorescence Imaging Plate Reader
The introduction of FLIPR™ (Fluorescence Imaging Plate Reader) in the 1990’s provided biologists with a fast and easy method of detecting G-protein coupled receptor (GPCR) activation through changes in intracellular calcium (Ca2+) concentration.

How does FLIPR work?

FLIPR Calcium Assay Kits utilize a calcium sensitive dye that is absorbed into the cell’s cytoplasm during incubation. Upon ligand-receptor binding, intracellular calcium is released, binding with the dye, thereby increasing fluorescence intensity.

What is a Flipr assay?

FLIPR (Fluorescent Imaging Plate Reader) was developed to perform quantitative optical screening for cell-based kinetic assays. FLIPR incorporates an integrated design, including low-level optical detection, precise temperature control, and precise fluid handling, all in one package.

What is a calcium flux assay?

Fluo-8 Calcium Flux Assay Kit (ab112129) is a no-wash, fluorescence-based assay for detecting intracellular calcium mobilization in cells from mammals and other species. In the calcium flux assay protocol, cells are pre-loaded with Fluo-8 which can cross the cell membrane.

What is calcium flux?

Indo-1 is the ratio metric calcium indicator dye most commonly used in flow cytometry because of its shift in emission frequency when excited at a single wavelength. …

What is calcium Mobilisation?

Calcium mobilization assay is a cell-based second messenger assay to measure the calcium flux associated with Gq-protein coupled receptor activation or inhibition. The method utilizes a calcium sensitive fluorescent dye that is taken up into the cytoplasm of most cells.

What does calcium flux mean?

Calcium flux is a rapid and sensitive measure of cell activation whose utility could be enhanced with better techniques for data extraction. We describe a technique to monitor calcium flux by flow cytometry, measuring Fura Red calcium dye by ratiometric analysis.

How is calcium measured in cells?

Changes in intracellular calcium concentration can be measured using the calcium sensitive fluorescent ratiometric dye fura-2 AM. This method is a high throughput way to measure agonist mediated calcium responses.

What is the source of the calcium mobilized in response to action potentials?

The upstroke of the action potential is generated by calcium influx through Cav1. 3 calcium channels (22), resulting in a rise in intracellular calcium.

What is a ratiometric dye?

Ratiometric, or dual-wavelength, ion indicators are a subcategory of fluorescent dyes utilized for their ability to quantitatively measure intracellular ion concentrations.

How does Fura-2 am work?

Fura-2, an aminopolycarboxylic acid, is a ratiometric fluorescent dye which binds to free intracellular calcium. The use of the ratio automatically cancels out confounding variables, such as variable dye concentration and cell thickness, making Fura-2 one of the most appreciated tools to quantify calcium levels.