What are the different types of chromatographic techniques?

Types of chromatography. Column chromatography.

What is the separation technique of chromatography?

Chromatography is a process for separating components of a mixture. The different components of the mixture travel through the stationary phase at different speeds, causing them to separate from one another.

What are the 12 types of chromatography?

The twelve types are: (1) Column Chromatography (2) Paper Chromatography (3) Thin Layer Chromatography (4) Gas Chromatography (5) High Performance Liquid Chromatography (6) Fast Protein Liquid Chromatography (7) Supercritical Fluid Chromatography (8) Affinity Chromatography (9) Reversed Phase Chromatography (10) Two …

What is chromatography explain with diagram?

Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid (gas or solvent) called the mobile phase, which carries it through a system (a column, a capillary tube, a plate, or a sheet) on which a material called the stationary phase is fixed.

How many methods of separation are there?

chromatography: Involves solvent separation on a solid medium. distillation: Takes advantage of differences in boiling points. evaporation: Removes a liquid from a solution to leave a solid material. filtration: Separates solids of different sizes.

What are two applications of chromatography?

1) It is used to separate solution of coloured substances. 2) It is used in forensic sciences to detect and identify trace amount of substances in the contents of bladder and stomach. 3) It is used to separate small amount of products of chemical reaction.

What is the purpose of chromatography?

The objective of chromatography is to separate the various substances that make up a mixture. The applications range from a simple verification of the purity of a given compound to the quantitative determination of the components of a mixture.

Why silica gel is used in TLC?

Silica gel is by far the most widely used adsorbent and remains the dominant stationary phase for TLC. The surface of silica gel with the highest concentration of geminal and associated silanols is favored most for the chromatography of basic compounds because these silanols are less acidic.

What are the two phases of thin layer chromatography?

Chromatography works on the principle that different compounds will have different solubilities and adsorption to the two phases between which they are to be partitioned. Thin Layer Chromatography (TLC) is a solid-liquid technique in which the two phases are a solid (stationary phase) and a liquid (moving phase).

What are the four separation techniques used in chromatography?

Four separation techniques based on molecular characteristics and interaction t ype use mechanisms of ion exchange, surface adsorption, partition, and size exclusion. Other chromatography techniques are based on the stationary bed, including column, thin layer, and paper chromatogr aphy.

How are the different types of chromatographic techniques classified?

There are different kinds of chromatographic techniques and these are classified according to the shape of bed, physical state of mobile phase, separation mechanisms.

Where does the word chromatography come from?

Chromatography derives its name from two Greek words as; (chroma) meaning “color” and (graphy) meaning “writing”. Chromatography is the collective term for a familyof laboratory techniques for the separation of mixtures.

How is the preparative application of chromatography used?

It is the preparative application of chromatography. It is used to obtain pure chemical compounds from a mixture of compounds on a scale from micrograms up to kilograms using large industrial columns.