What is the restriction site for PstI?

Description. Thermo Scientific PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.

What is the original bacterial source of the PstI enzyme?

E. coli
Product Source A E. coli strain that carries the PstI gene from Providencia stuartii 164 (ATCC 49762).

What is pst1 restriction enzyme?

PstI is a restiction enzyme used in molecular biology applications to cleave DNA at the recognition sequence 5′-CTGCA/G-3′, generating DNA fragments with 3′- cohesive termini.

What does the EcoRI PstI enzyme mixture do?

Restriction enzymes work by recognizing a particular sequence of bases on the DNA. The enzyme then cuts the backbones of both strands, allowing the DNA to separate into two pieces. For example, the enzyme EcoRI (see the figure, left, top) binds to the recognition sequence GAATTC and cuts between the G and the A.

Is pstl a restriction enzyme?

PstI is a useful enzyme for DNA cloning as it provides a selective system for generating hybrid DNA molecules. These hybrid DNA molecules can be then cleaved at the regenerated PstI sites. It is also an isoschizomer restriction enzyme SalPI from Streptomyces albus P.

What are restriction endonucleases?

Restriction endonucleases (REs) are bacterial enzymes that cleave double-stranded DNA. REs are present in bacteria presumably to destroy DNA from foreign sources (e.g., infecting bacteriophage) by cleaving the foreign DNA at specific recognition sites.

What does PstI stand for?

PSTI

How many fragments did the restriction enzyme PstI produce?

c) For those two restriction endonucleases, each DNA fragment in the single digest is cut by PstI into two DNA fragments in the double digest (i.e. the restriction endonuclease plus PstI).

Is pst1 sticky or blunt?

Function. PstI cleaves DNA at the recognition sequence 5′-CTGCA/G-3′ generating fragments with 3′-cohesive termini. This cleavage yields sticky ends 4 base pairs long. PstI is catalytically active as a dimer.

What are examples of restriction enzymes?

SmaI is an example of a restriction enzyme that cuts straight through the DNA strands, creating DNA fragments with a flat or blunt end. Other restriction enzymes, like EcoRI, cut through the DNA strands at nucleotides that are not exactly opposite each other.

How many fragments are produced by BamHI?

Under ideal conditions there would be 6 fragments from Enzymes A and B, and 8 fragments from Enzyme C. GGATCC is the recognition site for BamHI and is found in λ DNA at 5 locations. GAATTC is the recognition site for EcoRI and is found in λ DNA at 5 locations.

Is the target sequence 5’CTGCAG the same as PstI?

Both enzymes recognize the target sequence 5’CTGCAG. The enzyme systems have similar methyltransferases (41% amino acid identity), restriction endonucleases (46% amino acid identity), and genetic makeup (58% nucleotide identity). These observations suggest a shared evolutionary history.

When to use Thermo Scientific PstI restriction enzyme?

Order requests are processed and shipped by your local distributor. Learn more Thermo Scientific PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI).

How many negatively charged residues are there in PstI?

It has a molecular weight of 69,500 and contains 54 positive and 41 negatively charged residues. The PstI restriction/modification (R/M) system has two components: a restriction enzyme that cleaves foreign DNA, and a methyltransferase which protect native DNA strands via histone methylation.

How is PstI used in restriction site mapping?

Its use is not limited to molecular cloning; it is also used in restriction site mapping, genotyping, Southern blotting, restriction fragment length polymorphism (RFLP) and SNP. It is also an isoschizomer restriction enzyme SalPI from Streptomyces albus P.