Guidelines

What type of test will show listeria monocytogenes?

What type of test will show listeria monocytogenes?

A blood test is often the most effective way to determine whether you have a listeria infection. In some cases, samples of urine or spinal fluid will be tested as well.

What are the tests used to confirm Listeria monocytogenes in food?

Immunological identification and confirmation tests based on latex agglutination, enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA) have been developed for Listeria spp. and lateral flow test strips have also been developed by several manufacturers using immunochromatographic technology.

How do you detect Listeria in the lab?

The characteristic motility of the organism in a liquid medium or semisolid agar is also helpful for the preliminary identification of Listeria. All gram-positive rods isolated from blood and CSF should be identified to distinguish between Corynebacterium (presumably a contaminant) and Listeria.

How is Listeria monocytogenes confirmed in food?

There are well established confirmation and identification procedures for Listeria spp and for L. monocytogenes. Preliminary identification based on colony appearance on chromogenic and other selective agar media is traditionally confirmed using classical biochemical and morphological tests.

What kind of test is used to diagnose Listeria?

This enhanced hemolysis is referred to as a positive CAMP (Christie, Atkins, Munch-Petersen) test. The characteristic motility of the organism in a liquid medium or semisolid agar is also helpful for the preliminary identification of Listeria.

What is the CAMP test for Listeria monocytogenes?

The CAMP (Christie, Atkins, Munch-Petersen) test must be interpreted with caution. Chromogenic media are based on both the specific chromogenic detection of phosphatidylinositol phospholipase C and the xylose fermentation and give specific and direct identification of L. monocytogenes and L. ivanovii.

How are chromogenic media used to identify Listeria?

Chromogenic media are based on both the specific chromogenic detection of phosphatidylinositol phospholipase C and the xylose fermentation and give specific and direct identification of L. monocytogenes and L. ivanovii. Isolates of L. monocytogenes with atypical properties require tools of molecular biology for final identification.