What cell type are Huvec cells?

Human umbilical vein endothelial cells (HUVEC) are primary cells isolated from the vein of the umbilical cord. They are a model system for studying endothelial cell function, with applications including hypoxia, inflammation, oxidative stress, response to infection, and both normal and tumor-associated angiogenesis.

What are Huvec cells used for?

Primary Human Umbilical Vein Endothelial Cells (HUVEC) are isolated from the vein of the umbilical cord and are commonly used for physiological and pharmacological investigations, such as macromolecule transport, blood coagulation, angiogenesis, and fibrinolysis.

Are human umbilical vein endothelial cells stem cells?

Human umbilical vein endothelial cells (HUVECs), a prominent subset of macrovascular endothelial cells, are commonly used to evaluate the function and pathology of endothelial cells (e.g., angiogenesis).

What are endothelial cells?

Endothelial cells are major participants in and regulators of inflammatory reactions. Resting endothelial cells prevent coagulation, control blood flow and passage of proteins from blood into tissues, and inhibit inflammation.

Are Huvec a cell line?

Human umbilical vein endothelial cells (HUVECs) are cells derived from the endothelium of veins from the umbilical cord. They are used due to their low cost, and simple techniques for isolating them from umbilical cords, which are normally resected after childbirth.

Are Huvec cells primary cells?

Primary Human Umbilical Vein Endothelial Cells (HUVEC) are isolated from human umbilical vein and cryopreserved at the end of the primary culture. Each vial contains over 0.5 x106 viable cells that have been cryopreserved at the end of the primary culture stage in a medium containing 10% DMSO.

Are Huvec immortalized?

Conclusion: Ectopic coexpression of hTERT and SV40 LT can effectively immortalize HUVECs without tumorigenicity in vitro. Immortalized HUVECs may be an ideal target of further molecular function studies.

What is human umbilical vein endothelial cells?

What foods are good for endothelial cells?

Preclinical studies have indicated that polyphenol-rich food and food-derived products such as grape-derived products, black and red berries, green and black teas and cocoa, and omega-3 fatty acids can trigger activating pathways in endothelial cells promoting an increased formation of nitric oxide and endothelium- …

Where is VEGF produced?

VEGF is produced by hypertrophic chondrocytes in the growth plate where it co-ordinates extracellular matrix (ECM) remodelling, angiogenesis, and bone formation. VEGF is expressed in the synovial fluid of patients with rheumatoid arthritis.

How are HUVEC cells cultured in clonetics?

HUVEC cells were a kind gift from Erik Thompson (Bernard O’Brien Institute of Microsurgery, Melbourne, Australia). HUVECs were cultured in Clonetics® Endothelial Cell Growth Medium (Lonza Australia Ltd., Australia). Passage number in all experiments was below 10.

How are haematopoietic growth factors used in HUVEC co culture?

Co-culture of monocytes on a HUVEC monolayer induced up to four cell divisions in a 9 day period. The PM response to haematopoietic growth factors (Flt3L, SCF, IL-6, IL-3 and M-CSF) was determined. M-CSF induced the greatest proliferative response in PM; IL-3 and Flt3L reduced basal and M-CSF-induced proliferation.

How are umbilical vein endothelial cells co-culture?

Human Umbilical Vein Endothelial Cells (HUVECs) Co-Culture with Osteogenic Cells: From Molecular Communication to Engineering Prevascularised Bone Grafts J Clin Med. 2019 Oct 3;8(10):1602.doi: 10.3390/jcm8101602. Authors

How are monocytes cultured for haematopoietic growth factors?

Briefly, monocytes were cultured in 96 well U-bottom plates at a concentration of 5 × 10 4 cells per well in 200 μL of media (α-MEM, 3% human serum (HS), 1% P/S, 2 mM L-GlutaMax™ ± M-CSF (5000 U/mL)). On day 4 of culture, cells were pulsed with 10 μL of an equimolar solution of BrdU and cytidine to give a final concentration of 300 μM.