Is R PE the same as PE?

R-Phycoerythrin (also known as PE or R-PE) is useful in the laboratory as a fluorescence-based indicator for the presence of cyanobacteria and for labeling antibodies, most often for flow cytometry. Its use is limited in immunofluorescence microscopy due to its rapid photobleaching characteristics.

What color is PE fluorescence?

red-orange
R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. It is excited by laser lines from 488 to 561 nm, with absorbance maxima at 496, 546, and 565 nm and a fluorescence emission peak at 578 nm.

What is PE Cy7?

PE-Cy7 (Ex-Max 496 nm/Em-Max 785 nm): is a tandem fluorochrome that combines PE and a cyanine dye. PE-Cy7 conjugated reagents are as bright as PE conjugates. However, PE-Cy7 is highly sensitive to photo-induced degradation resulting in loss of fluorescence.

Which is the best wavelength for Vio dyes?

With a maximum absorption and emission at 400 nm and 455 nm respectively, VioBlue Fluorochromes are fully compatible with standard filter sets from all major flow cytometry hardware providers, giving researchers the flexibility to use the VioBlue Dye with existing laboratory platforms (figure 2). Intensity Wavelength (nm) 330 380 430 A

Which is the best combination of PE and cyanine dyes?

PE-Cy7 (Ex-Max 496 nm/Em-Max 785 nm): is a tandem fluorochrome that combines PE and a cyanine dye. PE-Cy7 conjugated reagents are as bright as PE conjugates.

How to calculate the protein concentration of dye?

280) is used to determine the protein concentration in a sample. However, because fluorescent dyes also absorb at 280 nm, a correction factor must be used to adjust for amount of A 280contributed by the dye. The correction factor (CF) equals the A 280of the dye divided by the A maxof the dye.

Which is better Pacific blue or vioblue dye?

The VioBlue® Dye is a coumarin-based fluorochrome with excitation and emission wavelengths of 400 nm and 455 nm, respectively. It is a superior alternative to Pacific Blue™, Alexa Fluor® 405, or BD™ Horizon™ V450.